Large-scale preparation and biological activity of recombinant human parathyroid hormone |
| |
| Affiliation: | 1. Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing, 100083, China;2. Center for Lignocellulose Science and Engineering, Liaoning Key Laboratory Pulp and Paper Engineering, Dalian Polytechnic University, Dalian, 116034, China;1. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Belt and Road Joint Laboratory of Advanced Fiber and Low-dimension Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, PR China;2. School of Materials Science and Engineering, Shanghai University, Shanghai 200444, PR China;1. Rubber Technology Centre, Indian Institute of Technology, Kharagpur, 721302, India;2. Department of Biotechnology, Indian Institute of Technology, Kharagpur, 721302, India;3. School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, 721302, India |
| |
| Abstract: | Human parathyroid hormone (hPTH) has been bacterially expressed in bioreactors as cro-β-galactosidase-hPTH fusion protein. We have developed a large-scale purification scheme that exploits the pH-dependent differential solubility of hPTH and a two-step Chromatographie procedure. We demonstrate that in a number of assay systems, the recombinant material obtained by this procedure is biologically active. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
