Liquid-gel partitioning using Lipidex in the determination of polychlorinated biohenyls,napthalenes, dibenzo-p-dioxins and dibenzofurans in blood plasma |
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| Institution: | 1. Department of Medical Biochemistry and Biophysics, Karolinska Institutet,S-171 77 Stockholm, Sweden;2. Department of Environmental Chemistry, Wallenberg Laboratory, Stockholm University, S-106 91 Stockholm, Sweden;1. Risk Assessment Division, Environmental Health Research Department, National Institute of Environmental Research, Incheon 22689, South Korea;2. Department of Chemical Engineering, Kwangwoon University, Seoul 01897, South Korea;1. Centro Nacional de Aceleradores (CNA), Universidad de Sevilla. Junta de Andalucía, Consejo Superior de Investigaciones Científicas, Parque científico y tecnológico Cartuja, Thomas Alva Edison 7, 41092 Sevilla, Spain;2. Dpto. de Física Aplicada I, Escuela Politécnica Superior, Universidad de Sevilla, Virgen de África 7, 41011 Sevilla, Spain;1. Key Laboratory of the Three Gorges Reservoir Region’s Eco-Environment of Ministry of Education, Chongqing University, Chongqing 400044, China;2. The Key Laboratory of Water and Sediment Sciences, Ministry of Education, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China;3. The Beijing Innovation Center for Engineering Science and Advanced Technology (BIC-ESAT), Peking University, Beijing 100871, China;1. State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, China;2. State Key Laboratory of Solid Waste Reuse for Building Materials, Beijing Building Materials Academy of Sciences Research, Beijing 100041, China;3. Institute of Environment and Health, Jianghan University, Wuhan 430056, China |
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| Abstract: | A method was developed for the transfer of fat, polychlorinated biphenyls (PCBs), naphthalenes (PCNs), dibenzo-p-dioxins (PCDDs) and dibenzofurans (PCDFs) from blood plasma into the lipophilic gel Lipidex 5000. Subsequent elution of the gel separated about 70% of the fat from the analytes. Different adsorbents and activated charcoal were applied for further purification of the sample and separation of analytes. Identification and determination of the chlorinated compounds were made by gas chromatography with electron-capture detection (GC-ECD) or gas chromatography-mass spectrometry (GC-MS). Recoveries were studied by addition of Halowax 1014 and different congeners of PCBs, PCNs, PCDDs and PCDFs to 50 ml of plasma. The mean recoveries of the individual compounds studied were 72–99%. By using the liquid-gel partitioning technique emulsions were avoided. Concentrations of lipids in plasma obtained by the present method agreed well with the concentrations obtained using liquid-liquid partitioning with chloroform-methanol. |
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