Simultaneous determination of amphetamine,methamphetamine, 3,4-methylenedioxyamphetamine and 3,4-methylenedioxymethamphetamine in human hair by gas chromatography-mass spectrometry |
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| Affiliation: | 1. University of Warsaw, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland;2. University of Warsaw, MISMaP College, Banacha 2C, 02-097 Warsaw, Poland;3. Medical University of Warsaw, Department of Nephrology, Banacha 1, 02-097 Warsaw, Poland;1. Genentech, Drug Metabolism and Pharmacokinetics, 1 DNA Way, South San Francisco, CA 94080, United States;2. Genentech, Clinical Pharmacology, 1 DNA Way, South San Francisco, CA 94080, United States;3. Covance Laboratories, 3301 Kinsman Blvd., Madison, WI 53704, United States;1. Forensic Toxicology Laboratory, Forensic and Scientific Services, Health Support Queensland, Department of Health, 39 Kessels Road Coopers Plains, Queensland, 4120, Australia;2. Roadside Drug Testing Unit, Road Policing Command, Queensland Police Service, Queensland, Australia;1. Unidad de Gestión Clínica de Nefrología, Complejo Hospitalario Universitario de Granada, Granada, Spain;2. Unidad de Urgencias, Hospital de Alta Resolución de Guadix, Guadix, Granada, Spain;3. Departamento de Estadística e IO, Universidad de Jaén, Jaén, Spain;1. The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, 471003 Luoyang, PR China;2. School of Pharmacy, Chengdu University of Traditional Chinese Medicine, 611137 Chengdu, PR China;3. Medical College of Henan University of Science and Technology, 471003 Luoyang, PR China |
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| Abstract: | A procedure is presented for the simultaneous identification and quantification of amphetamine (AP), methamphetamine (MA), methylenedioxyamphetamine (MDA) and methylenedioxymethamphetamine (MDMA) in human hair. The method involves decontamination of hair with dichloromethane and warm water, heat-alkaline hydrolysis in the presence of deuterated internal standards, liquid-liquid extraction and gas chromatography-mass spectrometry after derivatization with pentafluoropropionic anhydride-pentafluoropropanol. The limit of detection for AP, MA and MDA was 0.05 ng/mg using a 50-mg hair sample; for MDMA it was 0.1 ng/mg. Coefficients of variation ranged from 7 to 18%. This assay has been successfully utilized in the evaluation of the deposition of the drugs in hair obtained from various parts of the anatomy of a stimulant abuser. |
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