The induction of dominant somatic mutations at the Dlb-1 locus |
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| Affiliation: | 1. Institute for High Energy Physics of National Research Center “Kurchatov Institute”, Protvino, Moscow Region, 142281, Russia;2. Moscow Institute of Physics and Technology (State University), Dolgoprudny, Moscow Region, 141701, Russia;1. Dipartimento di Ingegneria Industriale, Università degli Sudi di Parma, Parco Area delle Scienze 181/A, 43124 Parma, Italy;2. Centro de Ciências Computacionais, Universidade Federal do Rio Grande, Cx.P. 474, Rio Grande, RS 96201-900, Brazil;3. School of Engineering, Universidade Federal do Rio Grande, Cx.P. 474, Rio Grande, RS 96201-900, Brazil;4. Departamento de Engenharia Mecânica, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 425, Porto Alegre, RS 90.050-170, Brazil;1. Registro Nacional de Donantes de CPH, INCUCAI, CABA, Argentina;2. Presidencia, Instituto Nacional Único de Ablación e Implante (INCUCAI), CABA, Argentina;1. National Institute for Laser, Plasma and Radiation Physics, Bucharest, Euratom-MEdC Association, Romania;2. Faculty of Materials Science and Engineering, Warsaw University of Technology, Warsaw, Poland;3. Culham Centre for Fusion Energy, Euratom Association, Abingdon, UK;1. Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA, USA;2. NOAA/NESDIS/STAR, College Park, MD, USA;3. NASA Goddard Space Flight Center, Greenbelt, MD, USA;4. Earth System Science Interdisciplinary Center, University of Maryland, College Park, MD, USA;5. Department of Geography and Earth Sciences, Aberystwyth University, Aberystwyth, UK;6. Centre of Atmospheric and Ocean Studies, University of Allahabad, Allahabad, India |
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| Abstract: | In the small intestine of heterozygous mice (Dlb-1b/Dlba), the Dlb-1 allele results in a stainable epithelium. The mutation or loss of the dominant Dlb-1b allele in a stem cell results in a non-staining ribbon of cells on a villus of the small intestine. To determine if dominant mutations resulting in the gain of staining — the induction of a Dlb-1b-like allele — could also be detected, we examined Dlb-1a homozygous mice (SWR) 2 weeks after a single treatment with 250 mg/kg ethylnitrosourea. Mutations to the dominant allele should appear as brown ribbons on unstained villi. Such ribbons were observed in the treated group but not in controls. The mutant frequency was low compared to the frequency of Dlb-1a-like mutations reported at the Dlb-1-b allele in heterozygous mice. |
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