Induction of micronucleated erythrocytes by MEA,AET, WR-2721 and X-rays |
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Institution: | 1. Department of Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin;2. Department of Pediatrics, Children''s Hospital of Wisconsin, Milwaukee, Wisconsin;3. Department of Surgery, Children''s Hospital of Wisconsin, Milwaukee, Wisconsin;1. Laboratório de Pesquisa em Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Brazil;2. Laboratório de Imunologia Clínica e Experimental, Instituto de Pesquisas Biomédicas, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Brazil;1. MaREI Centre, Environmental Research Institute, University College Cork, Cork, Ireland;2. School of Engineering, University College Cork, Ireland;3. Key Laboratory of Low-Grade Energy Utilization Technologies and Systems, Chongqing University, Chongqing, 400044, China |
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Abstract: | The induction of micronucleated polychromatic erythrocytes (MNPCEs) was assessed in the bone marrow of adult male Swiss mice treated with MEA (cysteamine HCl), AET (2-aminoethylisothiouronium Br.HBr), or WR-2721 (S-2-(3-aminopropylamino)ethyl phosphorothioic acid), at a dose of 200 mg/kg body weight, and/or exposed to 6 Gy X-rays. MEA, AET, or WR-2721 was given alone or 15 min prior to X-ray exposure, and the frequency of MNPCEs was determined 24 h after the aminothiol treatment and X-irradiation of mice. A genotoxic effect was shown for MEA, AET, WR-2721, and X-rays, as well as a protective effect of the aminothiols against X-ray-induced genotoxicity in the mouse erythropoietic system. The aminothiol drugs given alone, without subsequent X-irradiation, elevated the frequency of MNPCEs, and WR-2721 appeared to be less toxic than AET and MEA. After exposure of mice to X-rays, the number of MNPCEs was distinctly increased. MEA, AET, or WR-2721 administration prior to X-irradiation resulted in a reduction of the X-ray-induced elevation of the frequency of micronuclei, but a stronger radioprotective effect was obtained following WR-2721 and AET treatment than after MEA application. So, the genotoxic and radioprotective effect of the aminothiols was dependent on the compound applied. |
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