Determination of the enantiomers of metoprolol and its major acidic metabolite in human urine by high-performance liquid chromatography with fluorescence detection |
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| Affiliation: | 1. Master''s Student in Engineering and Environmental Management, Universidad Surcolombiana, Neiva, Huila, Colombia;2. Research Group of Science, Engineering and Innovation, Crimarpez S.A.S, Calle 12 Sur N° 6-45, Neiva, Huila, Colombia;3. Corporación Universitaria Minuto de Dios, Programa de Administración en Seguridad y Salud en el Trabajo, Grupo de Investigación en Seguridad y Salud en el Trabajo, Neiva, Huila, Colombia;4. Universidad Surcolombiana, Faculty of Engineering, Agricultural Engineering Program, Hydro Engineering and Agricultural Development Research Group (GHIDA), Avenida Pastrana Borrero - Carrera 1, Neiva, Huila, Colombia;5. Universidad Nacional de Colombia, Bogotá Campus, Faculty of Sciences, Department of Pharmacy, Pharmaceutical-Physical-Chemical Research Group, Carrera 30 No. 45-03, Bogotá D.C., Colombia;6. Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz 51664, Iran;7. Kimia Idea Pardaz Azarbayjan (KIPA) Science Based Company, Tabriz University of Medical Sciences, Tabriz 51664, Iran;8. Universidad Cooperativa de Colombia, Department of Engineering, Industrial Engineering Program, GRIAUCC Research Group, Calle 11 No. 1 - 51, Neiva, Huila, Colombia;1. Department of Chemistry and Applied Chemistry, Graduate School of Science and Engineering, Saga University, Honjo-machi, Saga 840-8502, Japan;2. Department of Chemistry and Applied Chemistry, Faculty of Science and Engineering, Saga University, Honjo-machi, Saga 840-8502, Japan;3. Faculty of Liberal Studies, National Institute of Technology, Kumamoto College, 2659-2 Suya, Koshi, Kumamoto 861-1102, Japan;1. Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, 71526 Assiut, Egypt;2. Department of Chemistry, University of Marburg, Hans-Meerwein-Straße, 35032 Marburg, Germany;1. Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;2. Drug Applied Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz 51664, Iran;3. Grupo de Investigaciones Farmacéutico-Fisicoquímicas, Departamento de Farmacia, Facultad de Ciencias, Universidad Nacional de Colombia – Sede Bogotá, Cra. 30 No., 45-03, Bogotá, D.C., Colombia;4. Department of Chemistry, University of North Texas, Denton, TX 76203-5070, USA;5. Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz 51664, Iran;6. Kimia Idea Pardaz Azarbayjan (KIPA) Science Based Company, Tabriz University of Medical Sciences, Tabriz 51664, Iran |
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| Abstract: | Enantiomers of metoprolol and its acidic metabolite H 117/04 were determined in human urine by high-performance liquid chromatography (HPLC) with fluorometric detection after chiral derivatization. The carboxyl functional group of the major metabolite was blocked by esterification after solid-phase extraction, which helped to quantitate this compound from interfering substances. The assay method was validated. The recovery of (−)- and (+)-metoprolol from urine was 86.3–90.5%; and the recovery of the (−)- and (+)-acidic metabolite H 117/04 from urine was 74.4–83.9% at different concentrations. |
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