Determination of iodide in serum and urine by ion-pair reversed-phase high-performance liquid chromatography with coulometric detection |
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| Affiliation: | 1. Faculté de Pharmacie de Tours, Service de Chimie Analytique, 2 bis Boulevard Tonnellé, 37042 Tours Cedex, France;2. Service d''Explorations Fonctionnelles Endocriniennes, Hôpital Trousseau, 26 Avenue du Dr. A. Netter, 75012 Paris, France;3. Laboratoire de Biochemie, Hôpital Trousseau, 26 Avenue du Dr. A. Netter, 75012 Paris, France;4. Faculté de Pharmacie, Service de Physiologie, Rue Jean Baptiste Clément, 92296 Chatenay-Malabry Cedex, France;1. Centre for Free Radical Research, Department of Pathology, University of Otago, Christchurch, New Zealand;2. Department of Medicine, University of Otago, Christchurch, New Zealand;1. Department of Civil and Environmental Engineering, The Hong Kong University of Science and Technology, Hong Kong, China;2. State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, China;1. Department of Cardiovascular Medicine, The First Affiliated Hospital of Xi''an Jiaotong University, Xi''an, Shaanxi, China;2. Department of Cardiovascular Medicine, Affiliated to the First People''s Hospital of Chenzhou of University of South China, Chenzhou No. 1 People''s Hospital, The First Affiliated Hospital of Xiangnan University, Chenzhou, Hunan, China;1. Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, USA;2. Otto Loewi Research Center, Division of Pharmacology, Medical University of Graz, Austria;3. Tumor Initiation & Maintenance Program and NCI Cancer Centre Proteomics Facility, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, USA;4. Gottfried Schatz Research Center, Division of Molecular Biology and Biochemistry, Medical University of Graz, Austria;5. Molecular Neuropathology Section, Laboratory of Neurogenetics, National Institute on Aging, National Institutes of Health, Bethesda, MD, 20892, USA;6. Department Neurosciences, School of Medicine, University of California, San Diego, La Jolla, CA, 92093, USA;7. Department of Pathology, School of Medicine, University of California, San Diego, La Jolla, CA, 92093, USA |
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| Abstract: | An HPLC method is described for the determination of iodide in serum and urine using ion-pair chromatography with coulometric detection. After adding hexadecyltrimethylammonium chloride, the ions pairs formed with the iodide in the sample are extracted using an organic solvent. The solvent is then evaporated and the dry residue obtained is mixed with an appropriate volume of mobile phase so as to concentrate the sample prior to injection into the chromatograph. For a sample of 0.5 ml of serum, the method features a limit of detection (signal-to-noise ratio of 3) of 0.2 μgl−1, sufficient to be applied in paediatric assays for the diagnosis of both iodide deficiency and excess. |
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