A ratiometric fluorescent probe for rapid and specific detection of hypochlorite |
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| Authors: | Pu Chen Qingli Nie Yuting Yan Juncheng Yang Hai-Tao Feng Ben Zhong Tang |
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| Affiliation: | 1. AIE Research Center, Shaanxi Key Laboratory of Phytochemistry, College of Chemistry and Chemical Engineering, Baoji University of Arts and Sciences, Baoji, China;2. College of Chemistry & Materials Science, Northwest University, Xi'an, China;3. School of Science and Engineering, Shenzhen Institute of Aggregate Science and Technology, The Chinese University of Hong Kong, Shenzhen (CUHK-Shenzhen), Shenzhen, China |
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| Abstract: | Hypochlorite (ClO−), as a kind of essential reactive oxygen species, plays a crucial role in vitro and in vivo. Here, a ratiometric fluorescent probe ( TPAM ) was designed and constructed for sensing ClO− based on substituted triphenylamine and malononitrile, which exhibited obvious colour transfer from orange to colourless under daylight accompanied by noticeable fluorescence change from red to green in response to ClO−. TPAM could effectively monitor ClO− with the merits of fast response, excellent selectivity, high sensitivity and a low detection limit of 0.1014 μM. 1H NMR, mass spectra and theoretical calculations proved that ClO− caused the oxidation of the carbon–carbon double bond in TPAM , resulting in compound 1 and marked changes in colour and fluorescence. In addition, TPAM was utilized for imaging ClO− in living cells successfully with good photostability and biocompatibility. |
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| Keywords: | cell imaging fluorescent probe hypochlorite ratiometric |
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