Methods for reducing the ammonia in hybridoma cell cultures |
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| Affiliation: | 1. Laboratoire de Biochimie Médicale, Pr Nabet, Faculté de Médecine, 54500 Vandoeuvre les Nancy, et IBN Nancy, France;2. Laboratoire de Biotechnologie, CEA Cadarache, 13108 Saint Paul Lez Durance, France;1. Division of Metabolism and Children''s Research Center, University Children''s Hospital Zurich, Zurich 8032, Switzerland;1. High Magnetic Field Laboratory, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, 230031, PR China;2. University of Science and Technology of China, Hefei, Anhui 230036, PR China;3. Department of Neurosurgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230036, PR China;4. Anhui Province Key Laboratory of Brain Function and Brain Disease, Hefei, Anhui 230031, PR China;1. Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel;1. Institute of Cell Culture Technology, Bielefeld University, Bielefeld, Germany;2. IBG-1: Biotechnology; Bioprocesses and Bioanalytics, Research Center Jülich, Germany;3. Bioinformatics Resource Facility, Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany;1. Project Planning & Coordination Department, Project & Lifecycle Management Unit, Chugai Pharmaceutical Co., Ltd., 1-1 Nihonbashi-Muromachi 2-Chome, Chuo-ku, Tokyo 103-8324, Japan;2. Human Resource Management Department, Chugai Pharmaceutical Co., Ltd., 1-1 Nihonbashi-Muromachi 2-Chome, Chuo-ku, Tokyo 103-8324, Japan;3. API Process Development Department, Pharmaceutical Technology Division, Chugai Pharmaceutical Co., Ltd., 5-1 Ukima 5-Chome, Kita-ku, Tokyo 115-8543, Japan;4. Utsunomiya Plant Manufacturing, Chugai Pharmaceutical Manufacturing Co., Ltd., 16-3 Kiyohara Kogyo Danchi, Utsunomiya, Tochigi 321-3231, Japan;5. Life Science and Bioengineering, Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1 Tennodai 1-chome, Tsukuba, Ibaraki 305-8572, Japan;1. Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1, Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-8530, Japan;2. Natural Science Center for Basic Research and Development, Hiroshima University, 1-3-1, Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-8530, Japan;3. Nanjing University of Aeronautics and Astronautics College of Material Science and Technology, No.29, Jiangjun Street, Nanjing, 211106, PR China |
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| Abstract: | The factors which limit the proliferation of eukaryotic cells in vitro are still not well known. Ammonia is believed to be toxic for mammalian cell proliferation and secretion. We have tried two approaches to reducing the ammonia in the medium. We first limited the ammonia produced by the cells by replacing glutamine by glutamate. Then, we used two chemical engineering methods to eliminate accumulated ammonia. In one the used medium was passed through a natural cation exchanger: the clinoptilolite. In the other, the culture medium was passed through a hydrophobic microporous hollow fiber module. Replacing the glutamine by glutamate reduced the medium ammonia concentration. The physicochemical removal of ammonia induced a better cell growth, but not a better specific antibody secretion. |
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