Simple fed-batch technique for high cell density cultivation of Escherichia coli |
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| Institution: | 1. Departamento de Procesos y Tecnología, Universidad Autónoma Metropolitana-Cuajimalpa, Mexico;2. Department of Biochemical Engineering, University College London, UK;3. RWTH Aachen University, AVT-Biochemical Engineering, Germany;1. Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, 35390 Giessen, Germany;2. Faculty of Biology and Chemistry, Justus-Liebig University of Giessen, 35390 Giessen, Germany;3. Fraunhofer Institute for Molecular Biology and Applied Ecology, Project Group Bioresources, 35390 Giessen, Germany;1. Division of Applied Chemistry, Graduate School of Engineering, Hokkaido University, N13W8 Kitaku, Sapporo 060-8628, Japan;2. CREST, Japan Science and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan;3. Institute of Life Technologies, University of Applied Sciences and Arts Western Switzerland (HES-SO Valais), Route du Rawyl 47, 1950 Sion, Switzerland;1. Christian Doppler Laboratory for Mechanistic and Physiological Methods for Improved Bioprocesses, Institute of Chemical Environmental and Bioscience Engineering, Vienna University of Technology, Vienna, Austria;2. Research Division Biochemical Engineering, Institute of Chemical Environmental and Bioscience Engineering, Vienna University of Technology, Vienna, Austria;1. Department of Automation, Kaunas University of Technology, Studentu 48, LT-51367 Kaunas, Lithuania |
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| Abstract: | A simple fed-batch process for high cell density cultivation of Escherichia coli TG1 was developed. A pre-determined feeding strategy was chosen to maintain carbon-limited growth using a defined medium. Feeding was carried out to increase the cell mass concentration exponentially in the bioreactor controlling biomass accumulation at growth rates which do not cause the formation of acetic acid (μ < μcrit). Cell concentrations of 128 and 148 g per 1 dry cell weight (g 1−1 DCW) were obtained using glucose or glycerol as carbon source, respectively. |
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