The comet assay: a comprehensive review |
| |
| Institution: | 1. Department of Electronics & Communication Engineering, National Institute of Technology Calicut, India;2. Department of Anatomy, Pondicherry Institute of Medical Sciences (PIMS), Kalapet Puducherry, India;3. Department of Anatomy, Jawaharlal Institute of Postgraduate Medical Education & Research (JIPMER), Puducherry, India;4. Department of Psychiatry, Jawaharlal Institute of Postgraduate Medical Education & Research (JIPMER), Puducherry, India;1. Department of Chemistry, Life Sciences and Environmental Sustainability, Parco Area delle Scienze 11/a, 43124 Parma, Italy;2. Centre for Molecular and Translational Oncology-COMT, University of Parma, Parco Area delle Scienze 11/a, 43124 Parma, Italy |
| |
| Abstract: | The comet assay is a sensitive and rapid method for DNA strand break detection in individual cells. Its use has increased significantly in the past few years. This paper is a review of the studies published to date that have made use of the comet assay. The principles of strand break detection using both the alkaline and neutral versions of the technique are discussed, and a basic methodology with currently used variations is presented. Applications in different fields are reviewed and possible future directions of the technique are briefly explored. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
