Aromatic ring oxidation of vanillyl and veratryl alcohols by Lentinus edodes: possible artifacts in the lignin peroxidase and veratryl alcohol oxidase assays |
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| Institution: | 1. Department of Biochemistry, Laboratory of Immunology, EDST-PRASE, Lebanese University, Faculty of Sciences, Hadath-Beirut, Lebanon;2. Laboratory of Experimental Hematology, Institut Jules Bordet, Université Libre de Bruxelles, 121, Boulevard de Waterloo, 1000 Bruxelles, Belgium;3. Innate Immune Signalling and Oncogenesis Group Lyon Cancer Research Center, Centre Léon Bérard, 28 rue Laennec, 69373 Lyon, France;1. Department of Physics, Sri Venkateswara University, Tirupati 517502, India;2. Department of Chemistry and Chemical Institute for Functional Materials, Pusan National University, Busan 609-735, Republic of Korea;3. Department of BIN Fusion Technology & Department of Polymer-Nano Science and Technology, Chonbuk National University, Jeonju, Jeonbuk, Republic of Korea;1. Taif University, Faculty of Science, Physics Department, Al-Haweiah, Taif, Saudi Arabia;2. Physics Department, Taibah University, Al-Madinah Al-Munawara, Saudi Arabia;3. Ain Shams University, Faculty of Science, Physics Department, Cairo, Egypt;4. Department of Engineering Physics and Mathematics, Faculty of Engineering, Tanta University, Egypt;1. Nanomagnetism and Nanotechnology Laboratory, International Islamic University, Islamabad 44000, Pakistan;2. Department of Material Science and Engineering, University of Science and Technology of China Hefei, Anhui 230026, China;3. Department of Physics, Quaid-e-Azam University, Islamabad 44000, Pakistan;4. Beijing National Laboratory for Condensed Matter Physics, National Laboratory for Superconductivity, Institute of Physics, Chinese Academy of Sciences, P.O. Box 603, Beijing 100190, China;5. Applied Thermal Physics Laboratory, Department of Physics, COMSATS Institute of Information Technology, Islamabad 44000, Pakistan;6. Magnetism Laboratory, Department of Physics, COMSATS Institute of Information Technology, Islamabad 44000, Pakistan |
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| Abstract: | The degradation pathway of vanillyl and veratryl alcohol by Lentinus edodes extracellular enzymes was studied. In both cases several products of side chain oxidation and aromatic ring cleavage were isolated and characterized. We have observed that the products from veratryl alcohol degradation by Lentinus edodes are quite different from those isolated from incubations with other white-rot fungi which have veraraldehyde as the major product, in fact, this compound is not produced as final metabolite in L. edodes incubations. This behaviour could explain the apparent absence of lignin peroxidase and veratryl alcohol oxidase activities in L. edodes cultures, since such activities are usually measured by monitoring veratraldehyde formation during the veratryl alcohol oxidation; thus, it is suggested that additional assay methods should be developed, with preferably direct observation of aromatic ring oxidation products. |
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