Determination of metronidazole in vaginal tissue by high-performance liquid chromatography using solid-phase extraction |
| |
| Affiliation: | 1. Department of Surgery and Cancer, Faculty of Medicine, Imperial College, London, UK;2. West London Gynecological Cancer Center, Queen Charlotte’s and Chelsea–Hammersmith Hospital, Imperial Healthcare NHS Trust, London, UK;3. Department of Obstetrics and Gynecology–Gynecological Oncology, University Hospital of Ioannina, Ioannina, Greece;4. Department of Obstetrics and Gynaecology, Worthing Hospital, Western Sussex Hospitals NHS Foundation Trust, Worthing, UK;5. IVF Unit, Queen Charlotte’s and Chelsea–Hammersmith Hospital, Imperial Healthcare NHS Trust, London, UK;6. Northern Gynecological Oncology Center, QE Gateshead NHS Trust, Gateshead, UK;1. Department of Obstetrics and Gynecology, Columbia University College of Physicians and Surgeons, United States;2. Department of Medicine, Columbia University College of Physicians and Surgeons, United States;3. Department of Radiation Oncology, Columbia University College of Physicians and Surgeons, United States;4. Department of Epidemiology, Mailman School of Public Health, Columbia University, United States;5. Herbert Irving Comprehensive Cancer Center, Columbia University College of Physicians and Surgeons, United States;6. New York Presbyterian Hospital, United States;1. National Cancer Institute, Bethesda, MD 20892, USA;2. Department of Pathology and Laboratory Medicine, Cumming School of Medicine, University of Calgary, Canada;3. ICF International, Inc., Rockville, MD 20878, USA;4. The American Society for Clinical Pathology Institute for Science, Technology, and Policy, Washington, DC 20005, USA;5. Mayo Clinic, Jacksonville, FL 32224, USA |
| |
| Abstract: | A sensitive high-performance liquid chromatographic (HPLC) method for the determination of metronidazole in vaginal tissue is reported. The method uses a Zorbax SB phenyl column with a 0.01 M aqueous monobasic potassium phosphate buffer (pH 4.0)-absolute methanol (85:15, v/v) as mobile phase at a flow-rate of 1.0 ml/min and detection at 313 nm. Tinidazole was used as the internal standard. The method employed homogenization of tissue followed by solid-phase extraction. The quantitation was achieved within 30 min with sensitivity in the ng/g range. Metronidazole was linear in the 100–2000 ng/g range. The accuracy and precision were in the 1–4% range for the drug and the limit of detection was approximately 100 ng/g based on a signal-to-noise ratio of 3 and a 100-μl injection. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
