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MOF@Au NPs/aptamer fluorescent probe for the selective and sensitive detection of thiamethoxam |
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| Authors: | Weiwei Yuan Shuhuai Li Xionghui Ma Chaohai Pang Yuwei Wu Mingyue Wang Bei Li |
| Affiliation: | 1. College of Food Science and Technology, and MOE, Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan, China Analysis and Test Center, Chinese Academy of Tropical Agricultural Sciences, Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables, Key Laboratory of Quality and Safety Control of Subtropical Fruits and Vegetables, Ministry of Agriculture and Rural Affairs, Haikou, China Hainan Institute for Food Control, Key Laboratory of Tropical Fruits and Vegetables Quality and Safety for State Market Regulation, Haikou, China;2. Analysis and Test Center, Chinese Academy of Tropical Agricultural Sciences, Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables, Key Laboratory of Quality and Safety Control of Subtropical Fruits and Vegetables, Ministry of Agriculture and Rural Affairs, Haikou, China;3. College of Food Science and Technology, and MOE, Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan, China;4. Hainan Institute for Food Control, Key Laboratory of Tropical Fruits and Vegetables Quality and Safety for State Market Regulation, Haikou, China |
| Abstract: | The luminescence performance of fluorescent reagents plays a crucial role in fluorescence analysis. Therefore, in this study, a novel bi-ligand Zn-based metal–organic framework, Au nanoparticle (NP) fluorescent material was synthesized using a hydrothermal method with Zn as the metal source. Simultaneously, a DNA aptamer was introduced as a molecular recognition element to develop a Zn-based MOF@Au NPs/DNA aptamer fluorescent probe for the ultra-trace detection of thiamethoxam residues in agricultural products. The probe captured different concentrations of the target molecule, thiamethoxam, through the DNA aptamer, causing a conformational change in the DNA aptamer and bursting the fluorescence of the probe, therefore establishing a fluorometric method for thiamethoxam detection. This method is highly sensitive due to the excellent luminescence properties of the Zn-based MOF@Au NPs, and the DNA aptamer can specifically recognize thiamethoxam, offering high selectivity. The linear range of the method was 2.5–6000 × 10−11 mol L−1, with a detection limit of 8.33 × 10−12 mol L−1. This method was applied to the determination of actual samples, such as bananas, and the spiked recovery rate was found to be in the range 84.05–109.07%. Overall, the proposed probe has high sensitivity, high selectivity, and easy operation for the detection of thiamethoxam residues in actual samples. |
| Keywords: | Au NPs DNA aptamer high sensitivity metal–organic framework thiamethoxam |