Development and validation of a high-sensitivity assay for an antipsychotic agent,CP-88,059, with solid-phase extraction and narrow-bore high-performance liquid chromatography |
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| Affiliation: | 1. Babson College, Babson Park, MA 02457, United States;2. King’s Business School, King’s College London, Bush House, 30 Aldwych, London WC2B4BG, United Kingdom;3. University of Bath, Claverton Down Road, Combe Down, Bath BA27AY, United Kingdom;1. Industry-Academic Cooperation Foundation, Hallym University College of Medicine, Seoul, 07247, South Korea;2. Department of Otolaryngology-Head and Neck Surgery, Kangbuk Samsung Hospital Sungkyunkwan University School of Medicine, Seoul, Republic of Korea;3. Department of Otorhinolaryngology-Head and Neck Surgery, Hallym University College of Medicine, Dongtan Sacred Heart Hospital, 7, Keunjaebong-gil, Hwaseong-si, 18450, South Korea;4. Department of Oral & Maxillofacial Surgery, Dentistry, Hallym University College of Medicine, Hallym Sacred Heart Hospital, Anyang, 14068, South Korea;5. Department of Neurosurgery, Hallym University College of Medicine, Dongtan Sacred Heart Hospital, 7, Keunjaebong-gil, Hwaseong-si, 18450, South Korea;1. School of Electromechanical and Automotive Engineering, Yantai University, Yantai 264005, China;2. Shi-changxu Innovation Center for Advanced Materials, Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, China;1. Division of Child Neurology, Department of Neurology, University of Washington School of Medicine and the Seattle Children''s Research Institute, Center for Integrative Brain Research, Seattle, Washington;2. Department of Pediatric Emergency Medicine, University of Washington, Seattle Children''s Hospital, Seattle, Washington;3. Department of Anesthesiology & Pain Medicine, University of Washington School of Medicine, Seattle, Washington;4. Center for Child Health, Behavior & Development, Seattle Children''s Research Institute, Seattle, Washington;5. Clinical Analytics, Seattle Children''s Hospital, Seattle, Washington |
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| Abstract: | An analytical method has been developed and validated for the quantitation of CP-88,059 in human serum. The compound and internal standard were extracted from serum by solid-phase extraction with a weak cation-exchange phase. The analytes were resolved from endogenous interferences using narrow-bore (2.1 mm I.D.) C18 reversed-phase HPLC. Column effluent was monitored by UV absorbance detection at 215 nm. The standard curve range was 1 to 250 ng/ml. The accuracy and precision values for the method were within ±10% and ±15%, respectively. A four-fold detectability enhancement was achieved using a 2.1 mm I.D. HPLC column relative to the more common 4.6 mm I.D. column. A performance comparison was made between the 2.1 mm I.D. column used for validation and a 4.6 mm I.D. column with the same stationary phase. |
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