Effect of ageing on human plasma glutathione concentrations as determined by high-performance liquid chromatography with fluorimetric detection |
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| Affiliation: | 1. Department of Medical Research, Taichung Veterans General Hospital, Taichung, 40705, Taiwan;2. Department of Food and Nutrition, Providence University, Taichung, 40705, Taiwan;1. Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea;2. Gachon Institute of Pharmaceutical Science, Gachon University, 191 Hambakmoero, Yeonsu-gu, Incheon 21936, Republic of Korea;3. College of Pharmacy, Gachon University, 191 Hambakmoero, Yeonsu-gu, Incheon 21936, Republic of Korea;1. Department of Chemistry and Physics, Indiana State University, Terre Haute, IN 47809 USA;2. Theoretical Chemistry Institute and Department of Chemistry, University of Wisconsin-Madison, WI 53706 USA;1. Physics Department, Ibb University, Ibb, Yemen;2. Faculty of Science, Menoufia University, Shebin El-Kom, Egypt;3. National Institute of Laser Enhanced Sciences, Cairo University, Giza, Egypt;1. Chemistry Department, Faculty of Science, Suez Canal University, 41522, Ismailia, Egypt;2. Chemistry Department, Faculty of Science, Arish University, El-Arish, Egypt |
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| Abstract: | A convenient method for the determination of reduced glutathione (GSH) and oxidized glutathione (GSSG) in human plasma by high-performance liquid chromatography with fluorescence detection is reported. This assay involves direct addition of human plasma to methanolic monobrombimane. for simultaneous protein precipitation and thiol derivatization. The assay was validated by addition of authentic GSH and GSSG to plasma samples. Plasma glutathione levels in Chinese male and female volunteers were found to decrease with increasing age (age groups, 20–30, 30–40, 40–50, 50–60, and >60; mean ± S.E.M. 0.97 ± 0.03, 0.77 ± 0.02, 0.67 ± 0.03, 0.51 ± 0.02, 0.48 ± 0.02 μM for male volunteers and 1.11 ± 0.06, 0.76 ± 0.03, 0.61 ± 0.03, 0.53 ± 0.04 and 0.43 ± 0.04 μM for female volunteers). GSSG levels, in both males and females, did not show a correlation with age. There were no significant differences in GSH or GSSG levels among male and female volunteers of the same age group. These results suggest that elderly persons might be more susceptible to oxidative injury due to decreased plasma glutathione levels. |
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