Genotoxic activity in vivo of the naturally occurring glucoside,cycasin, in the Drosophila wing spot test |
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| Institution: | 1. Faculty of Pharmaceutical Sciences, Meijo University, Yagotoyama 150, Tempaku-ku, Nagoya 468, Japan;2. Department of Pathology, Fujita-Gakuen Health University School of Medicine, I-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi 470-11, Japan;1. Program of Animal Science, Centro de Educação Superior do Oeste, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil;2. Department of Animal Science, Centro de Educação Superior do Oeste, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil;1. Graduate Program in Health Sciences, Center for Health Sciences, Federal University of Rio Grande do Norte, Natal, RN, Brazil;2. Graduate Program in Development and Environment, Biosciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil;3. Graduate Program in Biochemistry, Biosciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil;4. Geology Department, Center for Exact and Earth Sciences, Federal University of Rio Grande do Norte, Natal, RN, Brazil;5. Department of Microbiology and Parasitology, Biosciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil;6. Centro Nacional de Energia Nuclear, Poços de Caldas, MG, Brazil;7. Department of Cell Biology and Genetics, Biosciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil;1. Department of Chemistry, QOPNA and CESAM, University of Aveiro, 3810-193 Aveiro, Portugal;2. Department of Genetics and Biotechnology (DGB), University of Trás-os-Montes and Alto Douro (UTAD), Quinta de Prados, 5001-801 Vila Real, Portugal;3. Department of Biology and CESAM, University of Aveiro, 3810-193 Aveiro, Portugal;4. Animal and Veterinary Research Centre (CECAV) and Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal;5. Biosystems & Integrative Sciences Institute (BioISI), Faculty of Sciences, University of Lisboa, Lisboa, Portugal;6. Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB) & Department of Biology and Environment, University of Trás-os-Montes e Alto Douro, Apartado 1013, 5001-801 Vila Real, Portugal;7. Department of Biology & GreenUP-CitabUP, Faculty of Sciences, University of Porto, Rua Campo Alegre s/n, 4169-007 Porto, Portugal;1. Dpto. Física Aplicada, Facultad CC. Experimentales, University of Huelva, Campus de El Carmen s/n, 21007 Huelva, Spain;2. Dpto. Física Aplicada I. Escuela Politécnica Superior, University of Sevilla, C/Virgen de Africa 7, 41012 Sevilla, Spain;3. Universitat Politècnica de Catalunya, Instituto de Técnicas Energéticas, Campus Sud Edificio ETSEIB, Planta 0, Pabellón C, Av. Diagonal 647, 08028 Barcelona, Spain;1. Universidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claro – Avenida 24A, 1515, Bela Vista, 13506-900 Rio Claro, Brazil;2. UNIARARAS – Centro Universitário Hermínio Ometto, Araras - Avenida Dr. Maximiliano Baruto, 500, Jardim Universitario, 13607-339 Araras, Brazil;1. School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, 152 – 160 Pearse Street Trinity College Dublin, Dublin 2, Ireland;2. School of Pharmacy and Pharmaceutical Sciences, Trinity Biomedical Sciences Institute, 152 – 160 Pearse Street Trinity College Dublin, Dublin 2, Ireland;3. School of Biological and Health Sciences, Technology University Dublin, Dublin City Campus, Kevin St., Dublin 8 D08 NF82, Ireland;4. School of Chemistry, Trinity College Dublin, Dublin 2, Ireland |
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| Abstract: | Cycasin, methylazoxymethanol-β-glucoside, is a naturally occurring carcinogenic compound. The genotoxicity of cycasin was assayed in the Drosophila wing spot test. Cycasin induced small single and large single spots on feeding at 10 μmol/g medium. The presence of these spots indicates that cycasin is genotoxic in Drosophila melanogaster. Microorganisms which showed β-glucosidase activity for cleaving cycasin to toxic aglycon were isolated from gut flora of the Drosophila larvae. Consequently, the Drosophila wing spot test would be useful for mutagenicity screening of other naturally occurring glucosides. |
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