Simultaneous determination of sulphamonomethoxine and its N4-acetyl metabolite in blood serum by high-performance liquid chromatography with direct injection |
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| Institution: | 1. Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, 26300 Gambang, Kuantan, Malaysia;2. School of Chemical Sciences, Universiti Sains Malaysia, 11800 Pulau Pinang, Malaysia;3. School of Chemical Engineering, Universiti Sains Malaysia, Engineering Campus, 14300 Nibong Tebal, Pulau Pinang, Malaysia;1. Exponent Inc., 1010 14th Street, San Francisco, CA, 94114, USA;2. US Environmental Protection Agency Office of Research and Development, 1200 Pennsylvania Ave NW 8623R, Washington, DC, 20460, USA;3. The Dow Chemical Company, Toxicology & Environmental Research and Consulting, 1803 Building, Midland, MI, 48674, USA;4. BASF SE, Experimental Toxicology and Ecology, 67056, Ludwigshafen, Germany;5. US Environmental Protection Agency, National Health Environmental Effects Research Lab, 109 T.W. Alexander Drive, MD B105 05, Research Triangle Park, NC, 27711, USA;6. Bayer CropScience, P.O. Box 12014, RTP, NC, 27709, USA;7. Charles River Laboratories, Developmental and Reproductive Toxicology, 1407 George Road, Ashland, OH, 44805, USA;8. Charles River Laboratories International, 251 Ballardvale Street, Wilmington, MA, 01887, USA;1. College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China;2. National Energy R&D Center for Non-Food Biomass, China Agricultural University, Beijing 100193, China;3. Department of Agriculture, High Institute Agronomic and Veterinary, Faranah 131, Guinea;4. Department of Agronomy, Faculty of Agriculture, Gomal University, Dera Ismail Khan 29050, Pakistan;5. Sorghum Institute, Shanxi Academy of Agricultural Sciences, Jinzhong, Shanxi 030600, China;1. Division of BioAnalytical Chemistry, Amsterdam Institute of Molecular and Life Sciences (AIMMS), Vrije Universiteit Amsterdam, Amsterdam, the Netherlands;2. Van''t Hoff Institute for Molecular Sciences, University of Amsterdam, Amsterdam, the Netherlands;3. Centre for Analytical Sciences Amsterdam, Amsterdam, the Netherlands |
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| Abstract: | A high-performance liquid chromatographic method with direct injection has been developed for the simultaneous determination of sulphamonomethoxine and its N4-acetyl metabolite in serum of animals and fish. A HISEP shielded hydrophobic-phase column (15 cm × 4.6 mm I.D.), a mobile phase of 0.05 M citric acid–0.2 M disodium hydrogenphosphate-acetonitrile (70:15:15, v/v), and ultraviolet detection at 265 nm were used. The standard calibration curves in serum of chicken, pig, cattle, rainbow trout and yellowtail were linear over the range 0.5–20 μg/ml. The recoveries of sulphamonomethoxine and its N4-acetyl metabolite from all serum samples determined at different concentrations (0.5, 2.0 and 10.0 μg/ml) were 93–103% and 90–103%, respectively. The lowest measurable sulphamonomethoxine and N2-acetyl metabolite concentrations were 0.04 and 0.1 μg/ml, respectively, for all serum samples. |
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