Simultaneous determination of N2-(3-aminopropyl)biopterin (oncopterin), biopterin and neopterin by high-performance liquid chromatography with fluorescence detection |
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| Institution: | 1. Institute for Comprehensive Medical Science, School of Medicine, Fujita Health University, Toyoake, Aichi 470-11, Japan;2. Department of Natural Science Informatics, School of Informatics and Sciences, Nagoya University, Nagoya 464-01, Japan;1. Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils, College of Hunan Province, Hunan University of Science and Technology, Taoyuan Road, Yuhu District, Xiangtan 411201, China;2. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Donghu South Road 7, Wuhan 430072, China;3. State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Shuangqing Road 18, Haidian District, Beijing, China;4. Hunan Province Key Laboratory of Coal Resources Clean-utilization and Mine Environment Protection, Hunan University of Science and Technology, Taoyuan Road, Yuhu District, Xiangtan 411201, China;1. School of Biology, Food and Environment, Hefei University, Hefei, 230601, PR China;2. Anhui Province Key Laboratory of Environmental Toxicology and Pollution Control Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, PR China;3. Institute of Physical Science and Information Technology, Anhui University, Hefei, 230601, PR China;1. Department of Biomedical Engineering, Boston University, Boston, MA 02215, United States;2. Howard Hughes Medical Institute, Boston University, Boston, MA 02215, United States;3. Division of Metabolism and Children''s Research Center, University Children''s Hospital, CH-8032 Zurich, Switzerland;4. Neurophotonics Laboratory UMR 8250, Paris Descartes University, CNRS, Sorbonne Paris Cité, F-75006 Paris, France;5. UMR-S UL-INSERM U954 Nutrition-Genetics-Environmental Risk Exposure and Reference Centre of Inborn Metabolism Diseases, Medical Faculty of Nancy University and University Hospital Centre, Nancy, France;6. Nutrition and Metabolism Unit, Queen Fabiola Children''s University Hospital, Free University of Brussels (ULB), 1020 Brussels, Belgium;12. Department of Oncology, Children''s Research Center, University Children''s Hospital, CH-8032 Zurich, Switzerland;8. Rare Disease Initiative Zurich (radiz), Clinical Research Priority Program for Rare Diseases, University of Zurich, CH-8006 Zurich, Switzerland;9. Zurich Center for Integrative Human Physiology, University of Zurich, CH-8006 Zurich, Switzerland |
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| Abstract: | A high-performance liquid chromatographic method is described for the simultaneous determination of N2-(3-aminopropyl)biopterin (oncopterin, a newly found natural pteridine in urine from cancer patients), biopterin, and neopterin in urine. For the detection and quantification of the compounds, fluorometry was used. Using Develosil ODS K-5 and Develosil ODS HG-5 reversed-phase columns and a Nucleosil 100-5SA strong cation-exchange column, oncopterin, biopterin, and neopterin in urine were completely separated and assayed simultaneously by fluorescence detection. Similar values of oncopterin were obtained using each of the three columns, and the Develosil ODS K-5 reversed-phase column gave the most satisfactory separation. The sensitivity was high enough to measure 1 pmol of each pteridine. The HPLC method was highly reproducible. Our preliminary results indicate that oncopterin could be a most sensitive marker for cancer. |
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