Determination of BAY x 7195, a novel leukotriene D4 antagonist,in human plasma by high-performance liquid chromatography with post-column photo derivatisation and fluorescence detection |
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| Institution: | 1. Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi 221005, India;2. Department of Physics, Institute of Science, Banaras Hindu University, Varanasi 221005, India;3. Viral Research and Diagnostic Laboratory, Department of Microbiology, Institute of Medical Science, Banaras Hindu University, Varanasi 221005, India;1. State Key Laboratory of Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun 130012, China;2. State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China;3. Department of Electrical and Computer Engineering, Rice University, 6100 Main Street, Houston, TX 77005, USA;4. Department of Electrical Engineering and Photonics Research Institute, The Hong Kong Polytechnic University, Hong Kong, China |
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| Abstract: | Methods to determine plasma concentrations of the leukotriene D4 antagonist BAY x 7195 by HPLC with post-column photo derivatisation and fluorescence detection are described. Following dilution and centrifugation plasma supernatant is injected onto the HPLC system allowing the selective determination of the drug with a limit of quantitation (LOQ) of 10 μg/l (method A). Sensitivity was further enhanced to a LOQ of 0.6 μg/l by employing solid-phase extraction whereby the analyte concentration in the injection solution was increased (method B). Data on recovery, accuracy and precision of both methods throughout the working range are presented. BAY x 7195 is stable in plasma after repeated freeze-thaw cycles and upon storage at ?20°C for at least 13 months. Method A was applied to a clinical study with oral administration of 250 mg BAY x 7195 where ca. 1% of the maximum plasma concentrations still could be accurately and precisely quantified. Method B was employed to determine the drug in plasma after administration of 1 mg as aerosol. |
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