Determination of ticlopidine in human plasma by high-performance liquid chromatography and ultraviolet absorbance detection |
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| Institution: | 1. School of Economics, Southwestern University of Finance and Economics, Chengdu, Sichuan 611130, China;2. School of Economics and Finance, Xi''an Jiaotong University, Xi''an, Shaanxi 710061, China;1. Institute of Pharmacy, University of Regensburg, Regensburg, Germany;2. Hospital Pharmacy, University Hospital Regensburg, Regensburg, Germany;3. Dept. of Pharmacology, University of Regensburg, Regensburg, Germany;4. University of Leipzig, Integrated Research and Treatment Center (IFB) Adiposity Diseases, Leipzig, Germany;5. Dept. of Anaesthesiology and Intensive Care Medicine, University Hospital Leipzig, Leipzig, Germany;6. Dept. of Anaesthesiology, Intensive Care and Emergency Medicine, Pain Therapy, Bergmannstrost Hospital Halle, Halle, Germany |
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| Abstract: | A simple HPLC method has been developed for the determination of ticlopidine in human plasma. Plasma samples were buffered at pH 9 and extracted with n-heptane-isoamyl alcohol (98.5: 1.5, v/v). Imipramine was used as internal standard. Chromatography was performed isocratically with acetonitrile-methanol-0.05 M KH2PO4 (20:25:55, v/v) at pH 3.0 containing 3% triethylamine at a flow-rate of 1 ml/min. A reversed-phase column, Supelcosil LC-8-DB, 15 cm × 4.6 mm I.D., 5 μm particle size, was used. The effluent was monitored by UV absorbance detection at 235 nm. The method showed good accuracy, precision and linearity in the concentration range 5–1200 ng/ml. The limit of quantitation was 5 ng/ml, with a precision (C.V.) of 8.91%, which is the same as that achieved by other authors with a previously published GC-MS method. The procedure described in this paper is simple and allows the routine assessment of ticlopidine plasma concentration in pharmacokinetic studies following therapeutic doses in human subjects. |
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