Adenylate cyclase activity from Hirudo medicinalis segmental ganglia: modulation by calcium and calmodulin |
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| Affiliation: | 1. Department of Neuroscience, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, United States;2. Department of Discovery Chemistry, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, United States;3. Department of Structural Biology, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, United States;4. Department of Biochemical and Cellular Pharmacology, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, United States;1. Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, New York;1. Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, State University of New York, Buffalo, New York |
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| Abstract: | An adenylate cyclase activity stimulated by serotonin and calmodulin is present in the segmental ganglia of the leech Hirudo medicinalis. Removal of the endogenous calcium binding protein does not alter the responsiveness of the enzyme to serotonin. The calmodulin antagonist, trifluoperazine, drastically reduces the amine stimulatory effect on both intact and calmodulin-depleted membranes. We suggest that calmodulin-sensitive and serotonin-stimulated adenylate cyclase are, at least functionally, independent. |
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