Effect of adenosine triphosphate (ATP) on the secretion of insulin induced by tolbutamide in the absence of glucose

Our experiments were carried out on the isolated perfused rat pancreas. The effect of ATP on insulin secretion induced by tolbutamide (a hypoglycemic sulfonylurea) was studied in the absence of glucose. The addition of ATP (0.165 mmol/l) to the perfusion medium did not significantly modify the first phase induced by tolbutamide (0.4 mmol/l) but potentiated the second phase.