Tumor Necrosis Factor Receptor Expression in HIV-1-Infected CD4+ T Cells |
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Authors: | Didier Hober Donat de Groote Nathalie Vanpouille Isabelle Dehart Lu Shen Pierre Wattr Michle Maniez-Montreuil |
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Institution: | Didier Hober,Donat de Groote,Nathalie Vanpouille,Isabelle Dehart,Lu Shen,Pierre Wattré,Michèle Maniez-Montreuil |
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Abstract: | We investigated whether HIV-1 can regulate tumor necrosis factor receptor (TNFR) expression in SupT-1, a CD4 + T-cell line. The cells were infected with HIV-1 containing 1,000 cpm RT activity, as early as day 3 after infection and all along the culture the supernatant level of core protein p24 was >250 pg/ml, and on days 6 and 9 after infection, p24 was found in 10 % of the cells as determined by indirect immunofluorescence assay. The cells were growing without loss of viability. The study of TNFR expression was based on a microassay for measurement of binding of 125I-TNFα to cells, in which free and cell-bound ligand separation was performed by centrifugation through oil. Scatchard analysis of TNFα binding on days 6 and 9 after infection revealed a 90 % increase in the expression of high-affinity membrane receptors in HIV + SupT-1 culture compared with uninfected cells (mean +/-S.D. = 501 +/-148.5 vs. 263 +/-77.8 receptors/cell, n = 9, P< 0.001) with no change in dissociation constants (mean +/? S.D. = 4.36 +/?1.06 vs. 4.00 +/?1.12 × 10?10 m ). |
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Keywords: | TNFR HIV-1 scatchard analysis CD4+ T-cell line |
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