Degradation of a Polymerase Chain Reaction (PCR) Product by Heat-Stable Deoxyribonuclease (DNase) Produced from Yersinia enterocolitica |
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Authors: | Hiroshi Nakajima Ken-ichiro Itoh Eiji Arakawa Masanao Inoue Tadashige Mori Haruo Watanabe |
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Abstract: | When crude deoxyribonucleic acid (DNA) preparations by boiling were used for the polymerase chain reaction (PCR) from pathogenic and non-pathogenic Yersinia enterocolitica strains, the amplified products were degraded after their storage at 4 C. The degradation of products was prevented by the addition of ethylenediaminetetraacetate (EDTA) or treatment with proteinase K. These findings indicate that Y. enterocolitica produced heat-stable deoxyribonuclease (DNase). Proteinase K treatment would be recommended to prevent heat-stable DNase contamination in the DNA preparations for PCR from Y. enterocolitica strains. |
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Keywords: | yersinia enterocolitica detection method polymerase chain reaction (PCR) heat-stable deoxyribonuclease (DNase) |
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