Effect of hyaluronan on the elastase-type activity of human skin fibroblasts. |
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| Authors: | Emmanuelle Bernard William Hornebeck Ladislas Robert |
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| Affiliation: | 1. Department of Pharmaceutical Chemistry, College of Pharmacy, Jouf University, Sakaka, Aljouf 2014, Saudi Arabia;2. Department of Pharmaceutics, Faculty of Pharmacy, Universiti Teknologi MARA (UiTM) Selangor, Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia;3. Johar Institute of Professional Studies Lahore, Nabi Bux, Main Ferozpur Road, Punjab, Pakistan;4. Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Raiwind Road, 55150 Lahore, Pakistan;5. Department of Pharmacy, University of Malakand, Dir Lower, Chakdara, KPK, Pakistan;6. Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan;7. Department of Pharmacology and Dental Therapeutics, Faculty of Dentistry, Lincoln University College, Jalan Stadium, SS 7/15, Kelana Jaya, 47301 Petaling Jaya, Selangor, Malaysia;1. Nanotechnology and Advanced Materials Department, Materials and Energy Research Center, Tehran, Iran;2. Faculty of Chemistry and Chemical Engineering, Malek Ashtar University of Technology, Iran |
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| Abstract: | The influence of hyaluronan (HA) on the expression of human skin fibroblast elastase-type protease (HSFEp) (Homsy et al, 1988) was studied. At confluency of HSF cultures, hyaluronan increased the level of HSFEp in a time and dose-dependent fashion, Optimal effect was observed after 48 h of culture and at 2 mg/ml HA concentration; the stimulatory, effect of HA could be suppressed by 1 μM cycloheximide. The enhancement of enzyme biosynthesis by HA was dependent on cell proliferation but quasi invariant with HSF passage number (from 7-21). |
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| Keywords: | Fibroblasts hyaluronan elastase-type activity |
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