Application of spheroid culture to human hepatocytes and maintenance of their differentiation |
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Authors: | Jian Zhong Tong Sophie Sarrazin Doris Cassio Frdric Gauthier Fernando Alvarez |
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Institution: | Jian Zhong Tong,Sophie Sarrazin,Doris Cassio,Frédéric Gauthier,Fernando Alvarez |
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Abstract: | Summary— Human hepatocytes cultured with a hormonally defined medium on non-adherent poly-(2-hydroxyethyl methacrylate) coated surface were able to form spheroids. The maintenance of liver-specific functions was assessed by following secretion of albumin, transferrin and α-antitrypsin that were still detectable after 4 months of spheroidal culture. Moreover, cytochrome P-450 IA was induced by methylcholanthrene for up to 2 weeks. This cell system is very promising for long-term in vitro studies of human hepatocyte functions. |
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Keywords: | human hepatocyte spheroid culture differentiation cytochrome P-450 |
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