ICP-MS法测定广西金樱根及炮制品中22种金属元素

为建立广西金樱根药材中22种金属元素的电感耦合等离子体质谱(ICP-MS)分析方法,该文对不同产地药材及其炮制品中金属元素含量进行比较分析,评价其药材的质量和优选适当的炮制方法。同时,采用 ICP-MS 法测定22种金属元素含量,绘制其特征金属元素柱状图谱,并采用系统聚类分析和主成分分析对金樱根药材不同产地、不同炮制品的45个样品进行判别研究。结果表明:广西金樱根不同产地药材及其炮制品的45个样品中金属元素含量存在一定差异,其中Al元素含量偏高,经聚类分析和主成分分析将产地分为三类,主成分分析特征元素为Al、Pb、Ba、Zn、As、Sr。该研究通过金属元素在不同产地金樱根及炮制品中的变化规律,发现金樱根醋炙的炮制方法最优,为金樱根的临床安全应用及质量控制提供了理论依据和方法学参考。

Determination of 22 metal elements in the roots of Rosa laevigata and its processed products from different habitats of Guangxi by ICP-MS

To establish a quality control method for simultaneous analysis of 22 metal elements in the root of Rosa laevigata, collected from different origins, habitats and processed products by inductively coupled plasma mass spectrometry(ICP-MS), and to optimize a processing method in R. laevigata between different processed products. The samples were analyzed by ICP-MS and the histograms were established based on the characteristic metal elements. Discrimination study was carried out based on the hierarchical clustering analysis(HCA)and principal component analysis(PCA)of 45 samples collected from different origins, habitats and processed products, of which the results showed that the content of each metal element in the 45 samples of R. laevigata was existed variation among the nine districts, of which the content of Al was abundant. The 45 samples were classified into three clusters. The results of PCA confirmed the accuracy of HCA. Six main factors were selected by PCA, and the PCA results showed that Al, Pb, Ba, Zn, As and Sr were the characteristic elements in R. laevigata. The results displayed that the content variations of metal elements in different habitats of R. laevigata and its processed products, and the vinegar stir fry method is optimization processing. This study could provide a theory and technical support for clinical safety application and the process quality control of R. laevigata.