两粤黄檀乙醇提取物化学成分分析及抗炎活性研究

为了研究壮药两粤黄檀的化学成分及其抗炎活性,阐明其药理作用机制,该研究采用小鼠二甲苯致炎耳廓肿胀法、角叉菜胶致炎足肿胀急性炎症模型,观察不同剂量两粤黄檀乙醇提取物对小鼠耳廓肿胀度、肿胀率及足肿胀度的影响,并利用液相色谱-质谱(LC-MS)、热裂解气相色谱-质谱(PY-GC/MS)等技术分析提取物的化学成分,结合网络药理学方法对其抗炎活性成分以及作用机制进行初步研究。结果表明:(1)两粤黄檀乙醇提取物具有良好的抗炎活性,高剂量组(8 g·kg~(-1))可以显著抑制二甲苯致小鼠耳廓肿胀度、角叉菜胶致炎足趾肿胀,其抑制率均高于50%;(2)从两粤黄檀乙醇提取物中共鉴定得到60个化合物,包括毛蕊异黄酮、芒柄花黄素、血根碱、胡椒碱、β-谷甾醇等多种抗炎活性成分;(3)网络药理学分析显示,两粤黄檀发挥抗炎作用的核心靶点为PIK3CA、EGFR、MAPK、SRC、STAT3、CYP19A1、IL2、MAOA等,涉及c GMP-PKG、cAMP、Focal adhesion、Rap1等信号通路。该研究结果为两粤黄檀的进一步开发利用提供了重要参考。

Chemical constituents analysis and anti-inflammatory activities of ethanol extracts from Dalbergia benthamii

Dalbergia benthamii is a traditional Chinese medicine commonly used by Zhuang People in Guangxi, which was reported to possess a variety of biological activities. But their chemical constituents, anti-inflammatory activities and their possible pharmacological mechanism of D. benthamii were unclear yet. The acute inflammation of different doses of ethanol extracts of D. benthamii was observed by the mouse xylene induced ear edema and carrageenan induced paw edema models. Then, the chemical constituents analysis of the extracts were determined by liquid chromatography-mass spectrometry(LC-MS)and pyrolysis gas chromatography-mass spectrometry(PY-GC/MS). Their mechanism of anti-inflammatory activity was analyzed by network pharmacology at the same time. The results were as follows:(1)Compared with the model group, high dose group(8 g·kg^-1)of ethanol extracts of D. benthamii could significantly inhibit the xylene induced mouse ear edema, carrageenan induced hind paw edema, with inhibition rate over 50%.(2)There were sixty compounds identified from the extracts, including some reported anti-inflammatory active constituents, such as isoflavone mullein, formononetin, sanguine, piperine, beta-sitosterol, etc.(3)The network pharmacological analysis were shown that the anti-inflammatory effects of D. benthamii might be related to potential targets of PIK3CA, EGFR, MAPK, SRC, STAT3, CYP19A1, IL2 and MAOA, etc., which were mainly involving signaling pathways of cGMP-PKG, cAMP, Focal adhesion and Rapl. The results will provide an important reference for the further development and utilization of the plants of D. benthamii.