尖孢镰孢菌果胶裂解酶基因家族鉴定及侵染表达模式分析

[背景]番茄枯萎病是番茄生产中常见的土传真菌病害.[目的]为鉴定番茄枯萎病基因组果胶裂解酶基因家族,明确该基因家族在侵染过程表达模式.[方法]采用生物信息学方法鉴定了番茄枯萎病尖孢镰孢菌(Fusarium oxysporum f sp.Lycopersici)基因组内PEL基因家族,并分析了基因结构、染色体定位及三级结...

Characterization of pectate lyase gene family in Fusarium oxysporum f. sp. Lycopersici genome and expression mode during inoculation

[Background] Tomato fusarium wilt is a common soil born fungal disease in tomato production. [Objective] To identify pectate lyase gene family members in Fusarium oxysporum f. sp. Lycopersici genome and to clarify the expression mode after inoculation.[Methods] PEL family was identified, and the distribution, physical and chemical properties, gene structure, and tertiary structure prediction of PELs in F. oxysporm f. sp. Lycopersici genome were performed by bioinformatics. Expression mode of FoPEL1−16 in inoculated tomato roots were analyzed by RT-qPCR. [Results] There were 16 FoPELs in F. oxysporm f. sp. Lycopersici genome. The amino acid sequences were 163−548 aa in length with 16−21 aa signal peptides. The FoPEL genes were unevenly distributed on 7 chromosomes. 16 FoPELs were divided into four groups according to gene structure and conserved motifs. Evolutionary analysis showed that FoPELs clustered in four clades. Similar domain structure presented in the same family according to tertiary structure prediction. RT-qPCR analysis showed that FoPEL expression levels significantly increased during inoculation. [Conclusion] The pectate lyase existed in the form of gene family in F. oxysporm f. sp. Lycopersici genome, and the difference of gene structure indicated their function diversity. The expression levels of FoPEL were significantly increased during infection, which indicated that FoPEL was involved in the pathogenicity. The study provided a theoretical basis for pathogenic gene function and plant-pathogen interaction of F. oxysporium.