| 微生物高通量快速检测技术研究进展 |
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| 引用本文： | 尚玉婷,叶青华,刘振杰,吴清平,张菊梅. 微生物高通量快速检测技术研究进展[J]. 微生物学通报, 2021, 48(8): 2801-2814 |
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| 作者姓名： | 尚玉婷 叶青华 刘振杰 吴清平 张菊梅 |
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| 作者单位： | 广东省科学院微生物研究所 华南应用微生物国家重点实验室 广东省微生物安全与健康重点实验室 广东 广州 510070;江南大学食品学院 食品科学与技术国家重点实验室 国家功能食品工程技术研究中心 食品安全与质量控制协同创新中心 江苏 无锡 214122 |
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| 基金项目： | 国家重点研发计划（2019YFC1606300） |
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| 摘 要： | 微生物在大自然中广泛存在,特别是食源性致病菌是引发食源性疾病的主要因素,传统的检测技术已难以适应疾病预防控制和基层监管执法的需求,因此更加快速灵敏的高通量检测技术成为研究的热点.在对微生物快速准确识别方面,主要包括双功能抗体、核酸适配体筛选和噬菌体鉴定等方法.在多目标检测方面,主要包括多重PCR、基质辅助激光解吸电离飞...
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| 关 键 词： | 微生物 高通量 快速检测技术 |
| 收稿时间： | 2020-12-23 |
Research progress on high-throughput rapid detection technology of microorganism |
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| SHANG Yuting,YE Qinghu,LIU Zhenjie,WU Qingping,ZHANG Jumei. Research progress on high-throughput rapid detection technology of microorganism[J]. Microbiology China, 2021, 48(8): 2801-2814 |
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| Authors: | SHANG Yuting YE Qinghu LIU Zhenjie WU Qingping ZHANG Jumei |
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| Affiliation: | Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, Guangdong 510070, China;State Key Laboratory of Food Science and Technology, National Engineering Research Center for Functional Food, Synergetic Innovation Center of Food Safety and Quality Control, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China |
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| Abstract: | Microorganism is ubiquitous in nature, especially foodborne pathogens that cause foodborne diseases. Traditional methods have been already difficult to fit the demands of disease prevention and supervision in the routine work of regulatory authority, so rapid and sensitive high-throughput detection technique has become a research hot spot. Based on the rapid and accurate recognition of microorganism, bi-functional antibodies, aptamers- and phage-based assays are introduced. In terms of multi-target detection, there are three methods that include multiplex PCR, matrix-assisted laser desorption ionization-time of flight mass spectrometry, and biochip. This paper systematically discussed the detection mechanism, advantages and disadvantages, and research progress of each method, hoping to provide theoretical guarantees and reference for the development of rapid high-throughput method for detecting microorganism in the future. |
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| Keywords: | microorganism high-throughput rapid detection technology |
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