Macronuclear DNA demethylation is involved in the encystment process of the ciliate Colpoda inflata. |
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| Authors: | Gemma Palacios A Martin-Gonzalez Juan C Gutierrez |
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| Institution: | 1. Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA, USA;2. Department of Biology, University of Massachusetts, Amherst, MA, USA;1. Université Paris Saclay, Faculté des Sciences d''Orsay, CNRS UMR 3347, INSERM, U1021, Orsay, France;2. Institut Curie, PSL Research University, CNRS UMR 3347, INSERM U1021, F-91405, Orsay, France;3. Systems Biology, Harvard Medical School, Boston, MA, 02115, USA;4. Marine Biological Laboratory, Woods Hole, MA, 02543, USA;5. Institut Universitaire de France, F-75005, Paris, France;6. Technical University of Munich, Germany |
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| Abstract: | Ciliate encystment is an eukaryotic cell differentiation process which involves a specific gene expression, to form the resting stage. In this study, we investigate, for first time, the DNA methylation pattern changes during encystment in the ciliate Colpoda inflata, and the 5-azacytidine effect on growing cells and encystment. Results indicate that 5-methylcytosine is present in macronuclear DNA of this ciliate and the 5-azacytidine treatment induces encystment in growth conditions. From restriction enzyme digestion and 5-azacytidine experiments, we conclude that a specific DNA demethylation is probably involved in the encystment gene expression of this ciliate. |
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