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苹果渣乙醇提取物抗菌活性及防腐作用研究
引用本文:张华玲,韩 静,刘 绪,蒲 柳,管媛媛,段洁莹. 苹果渣乙醇提取物抗菌活性及防腐作用研究[J]. 广西植物, 2021, 41(7): 1181-1187
作者姓名:张华玲  韩 静  刘 绪  蒲 柳  管媛媛  段洁莹
作者单位:成都师范学院 化学与生命科学学院,成都 611130;酿酒生物技术及应用四川省重点实验室,四川 自贡 643000;成都师范学院 化学与生命科学学院,成都 611130
基金项目:酿酒生物技术及应用四川省重点实验室开放基金项目(NJ2017-13); 国家大学生创新创业项目(201814389142); 四川省科技计划重点研发项目(2019YFG0170)[Supported by Liquor-Makign Bio-Technology & Application of Key Laboratory of Sichuan Province(NJ2017-13); College Students Innovation and Entrepreneurship Program(201814389142); Key R & D Projects of Sichuan Science and Technology Program(2019YFG0170)]。
摘    要:为提高苹果渣资源利用率,探究苹果渣乙醇提取物的抗菌活性和防腐性能,该文采用微波辅助提取法制取苹果渣乙醇提取物,用抑菌圈实验测定其抗菌活性,并研究了其防腐作用.结果表明:(1)苹果渣乙醇提取物对酵母菌抑制作用不明显(抑菌圈直径<1 mm),对金黄色葡萄球菌和大肠杆菌的抑菌作用较明显(抑菌圈直径为6~9 mm),最佳抑菌浓...

关 键 词:苹果渣  乙醇提取物  百香果  抗菌活性  防腐作用
收稿时间:2020-11-03

Antibacterial activity and antiseptic effect of ethanol extract from apple pomace
ZHANG Hualing,HAN Jing,LIU Xu,PU Liu,GUAN Yuanyuan,DUAN Jieying. Antibacterial activity and antiseptic effect of ethanol extract from apple pomace[J]. Guihaia, 2021, 41(7): 1181-1187
Authors:ZHANG Hualing  HAN Jing  LIU Xu  PU Liu  GUAN Yuanyuan  DUAN Jieying
Affiliation:1. College of Chemistry and Life Sciences, Chengdu Normal University, Chengdu 611130, China; 2. Liquor-Makign Bio-Technology & Application of Key Laboratory of Sichuan Province, Zigong 643000, Sichuan, China
Abstract:In order to improve the utilization of apple pomace resources, verify the antibacterial activity and antiseptic properties of the ethanol extract of apple pomace, the antibacterial activity of extract from apple pomace were determined by the inhibition zone test. In addition, the antibacterial effect of the extract under different pH and salt concentration had been studied. At the same time, the ethanol extract of apple pomace was applied to the preservation of Passiflora edulia, using sensory scores and various physical and chemical indicators(Vc, soluble solids, total acid, solid acid ratio)as the basis to investigate the antiseptic effect of the extract. The results were as follows:(1)The ethanol extract of apple pomace had no obvious inhibitory effect on yeasts(diameter of inhibition zone <1 mm), but it had obvious inhibitory effect on Staphylococcus aureus and Escherichia coli(diameter of inhibition zone was 6-9 mm).(2)The best inhibitory concentration for these strains was 4.0 g·L-1. In addition, when the antibacterial effect of the extract was optimal, the pH was 6-7 and the salt concentration was 5.0 g·L-1.(3)The optimal concentration for Passiflora edulia was 0.2%, and the rot rate after storage at this concentration was only 6.7%(control group was 67%), and the weight loss rate was 5.5%(control group was 36.3%). The soluble solids and total acid content were not significantly different from those before storage(P>0.05)(control group P<0.05). And most of the fruits were plump, firm, bright and shiny, moderately sweet and sour. The antibacterial extract of apple pomace has antibacterial activity and significant antiseptic effect, and provides a theoretical basis for the development and utilization of apple pomace resources.
Keywords:apple pomace   ethanol extracts   Passiflora edulia   antibacterial activity   antiseptic effect
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