Structural analysis of the alpha-D-glucan (EPS180) produced by the Lactobacillus reuteri strain 180 glucansucrase GTF180 enzyme |
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Authors: | van Leeuwen Sander S Kralj Slavko van Geel-Schutten Ineke H Gerwig Gerrit J Dijkhuizen Lubbert Kamerling Johannis P |
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Institution: | Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands. |
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Abstract: | The neutral exopolysaccharide EPS180 produced from sucrose by the glucansucrase GTF180 enzyme from Lactobacillus reuteri 180 was found to be a (1-->3,1-->6)-alpha-D-glucan, with no repeating units present. Based on linkage analysis, periodate oxidation, and 1D/2D 1H and 13C NMR spectroscopy of the intact EPS180, as well as MS and NMR analysis of oligosaccharides obtained by partial acid hydrolysis of EPS180, a composite model, that includes all identified structural features, was formulated as follows: Formula: see text]. |
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Keywords: | d-Glucan" target="_blank">α-d-Glucan Polysaccharide structural analysis GTF180 Glucansucrase 1H NMR spectroscopy Lactobacillus reuteri 180 |
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