一株海洋细菌产碱性蛋白酶的研究

对1株产碱性蛋白酶的海洋细菌Pseudomonas sp.7-11发酵产生碱性蛋白酶的条件和酶的调控机制进行了初步研究。结果显示，7－11为好氧菌，具有嗜低温特性，且适宜在偏碱性的条件下生长并产酶，该菌产生碱性蛋白酶表现出初级动力学持征；酷蛋白培养基适合菌株产酶；K^ 对于菌株产酶有关键作用；复合氨基酸对产酶有抑制作用；初步显示，7－11产生蛋白酶受诱导及阻遏两种方向的调控。

Study on the production of an alkaline proteaseproduced by a marine bacterium

The fermentation conditions of the alkaline protease produced by a marine bacterium strain 7-11 and the regulation mechanism of the protease production was studied.The study showed that 7-11 was aerobic and psychrophilic.The curve of alkaline protease production showed primary metabolite kinetics.the casein medium improved the growth of the organism and the production of the protease.K was vital for the enzyme production.Composite amino acid inhibited the production of the protease.