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左肾动脉缩窄诱导心肌肥大大鼠心肌蛋白差异表达研究
作者姓名:Lv YY  Sun B  Ma JZ
作者单位:[1]南京体育学院运动人体科学系,江苏南京210014; [2]解放军理工大学军事教育训练系,江苏南京210000
基金项目:江苏省体育局基金资助项目(TY6109)
摘    要:目的:应用蛋白质组学方法分析病理性心肌肥大心肌细胞蛋白表达特征。方法:雄性SD大鼠16只,分为2组(n=8):两肾一夹组(2K1C)和假手术组(SO),术后饲养8周,使用普通多普勒和组织多普勒鉴定动物模型,然后提取心肌总蛋白,应用二维凝胶电泳技术,建立分辨率高和重复性良好的凝胶图像,质谱(MALDI—TOF-MS)鉴定差异蛋白点,与网络数据库进行匹配,并对鉴定蛋白进行分类。结果:两肾一夹大鼠心肌细胞有21个蛋白点表现出显著增加或减少,经数据库匹配,获得14种差异表达的蛋白质。结论:两肾一夹大鼠心肌出现一些差异表达蛋白席官们可能在病理性心肌肥大的发生中起重要作用。

关 键 词:蛋白质组  二维电泳  质谱  心肌肥大  肾动脉缩窄

Proteomic analysis of myocardial hypertrophy induced by left kidney artery coarctation in rats
Lv YY,Sun B,Ma JZ.Proteomic analysis of myocardial hypertrophy induced by left kidney artery coarctation in rats[J].Chinese Journal of Applied Physiology,2009,25(2):237-242.
Authors:Lv Yuan-yuan  Sun Biao  Ma Ji-zheng
Institution:Department of Kinesiology, Nanjing Sport Institute, Nanjing 210014, China. qi-huang006@foxmail.com
Abstract:To identify the expression of proteins in cardiomyocytes in rats with left kidney artery coarctation. Methods: 16 male SD rats were separated into 2 groups( n = 8) : 2 kidney 1 Clip group(2K1C) and sham operation group(SO). The postoperational 8th week, after examination by normal doppler and tissue doppler echocardiography, the extracted proteins from cardiomyocytes were isolated by two-dimensional gel electrophoresis with staining. The gel images were acquired by scanner and 2-DE analysis software. Different spots observed on two 2D gels were selected and identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF-MS). Results: Overall, 21 protein spots showed significant difference, and 14 out of which were identified. Conclusion: Kidney artery coactation-induced cardiac hypertrophy displays different expression of proteins in cardiomyocytes.
Keywords:proteomics  two-dimensional electrophoresis  mass spectrometry  cardiac hypertrophy  kidney artery coarctation
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