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Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2) |
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| Authors: | Gabriel C. Uguru Madhav Mondhe Shan Goh Andrew Hesketh Mervyn J. Bibb Liam Good James E. M. Stach |
| Affiliation: | 1. School of Biology, Newcastle University, Newcastle upon Tyne, United Kingdom.; 2. Department of Pathology and Infectious Diseases, Royal Veterinary College, University of London, London, United Kingdom.; 3. John Innes Centre, Norwich, United Kingdom.; Center for Genomic Regulation, Spain, |
| Abstract: | We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production. Synthetic RNA silencing was target-specific and is a new tool for gene regulation and metabolic engineering studies in Streptomyces. |
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