| 基于CRISPR的快速灵敏便捷分子检测 |
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| 引用本文： | 孙雯君,黄行许,王鑫杰.基于CRISPR的快速灵敏便捷分子检测[J].生物工程学报,2023,39(1):60-73. |
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| 作者姓名： | 孙雯君 黄行许 王鑫杰 |
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| 作者单位： | 上海科技大学生命科学与技术学院, 上海 201210;上海科技大学生命科学与技术学院, 上海 201210;广州生物岛实验室, 广东 广州 510030;中国农业科学院(深圳)农业基因组研究所 岭南现代农业科学与技术广东省实验室深圳分中心 农业农村部农业基因数据分析重点实验室, 广东 深圳 518120;广州生物岛实验室, 广东 广州 510030 |
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| 基金项目： | 国家自然科学基金(82002144);广州实验室应急攻关项目(EKPGL2021018);上海市科委基金(21N31900400) |
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| 摘 要： | 快速灵敏检测技术对疾病防控必不可少。特别是新冠疫情暴发以来,人们深刻认识到快速灵敏检测技术的重要性。近年来,以CRISPR/Cas为代表的基因编辑技术带来了生物技术革命性的进步。CRISPR的核酸检测技术因其快速、准确、灵敏、经济等特点,正在引发分子诊断革新,并已被成功应用于传染病、遗传病、肿瘤基因突变诊断,以及食品安全等领域。本文归纳了基于CRISPR的多种核酸检测体系及应用,并对未来CRISPR核酸检测发展趋势及结合人工智能的智能化检测进行了展望。
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| 关 键 词： | 分子诊断 核酸检测 临场检测 基因编辑 CRISPR CRISPR检测 |
| 收稿时间： | 2022/4/18 0:00:00 |
| 修稿时间： | 2022/8/2 0:00:00 |
CRISPR-based molecular diagnostics: a review |
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| SUN Wenjun,HUANG Xingxu,WANG Xinjie.CRISPR-based molecular diagnostics: a review[J].Chinese Journal of Biotechnology,2023,39(1):60-73. |
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| Authors: | SUN Wenjun HUANG Xingxu WANG Xinjie |
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| Institution: | School of Life Science and Technology, Shanghai Tech University, Shanghai 201210, China;School of Life Science and Technology, Shanghai Tech University, Shanghai 201210, China;Guangzhou Laboratory, Bio-Island, Guangzhou 510030, Guangdong, China; Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, Guangdong, China;Guangzhou Laboratory, Bio-Island, Guangzhou 510030, Guangdong, China |
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| Abstract: | Rapid and accurate detection technologies are crucial for disease prevention and control. In particular, the COVID-19 pandemic has posed a great threat to our society, highlighting the importance of rapid and highly sensitive detection techniques. In recent years, CRISPR/Cas-based gene editing technique has brought revolutionary advances in biotechnology. Due to its fast, accurate, sensitive, and cost-effective characteristics, the CRISPR-based nucleic acid detection technology is revolutionizing molecular diagnosis. CRISPR-based diagnostics has been applied in many fields, such as detection of infectious diseases, genetic diseases, cancer mutation, and food safety. This review summarized the advances in CRISPR-based nucleic acid detection systems and its applications. Perspectives on intelligent diagnostics with CRISPR-based nucleic acid detection and artificial intelligence were also provided. |
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| Keywords: | molecular detection nucleic acid detection point-of-care testing (POCT) gene editing CRISPR CRISPR-based detection |
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