| 蓝细菌Anacystis nidulans R-2藻胆体中43 Kd蛋白细胞定位的初步研究 |
| |
| 引用本文： | 李荣贵,汪靖超. 蓝细菌Anacystis nidulans R-2藻胆体中43 Kd蛋白细胞定位的初步研究[J]. 植物学通报, 2005, 22(3): 302-306 |
| |
| 作者姓名： | 李荣贵 汪靖超 |
| |
| 作者单位： | 青岛大学生物系,山东省天然色素重点实验室,青岛266071 |
| |
| 基金项目： | 国家自然科学基金项目(39525002,39570067)资助. |
| |
| 摘 要： | 高盐浓度条件下分离了蓝细菌Anacystis nidulans R-2的藻胆体，藻胆体中存在一种43kD的蛋白。Western blotting分析表明，该蛋白能与蓝细菌Fd：NADP氧还酶中FNRE占构域的抗体发生反应，解聚的藻胆体具有FNR黄递酶的活性，初步证明该43kD蛋白就是Fd：NADP氧还酶。Triton X-114分相实验表明，这种43kD的蛋白不能进入Triton X-114相。对藻胆体的部分解聚合实验表明，富含外周杆的组分中不存在43kD的蛋白。
|
| 关 键 词： | 43kD蛋白 藻胆体 蓝细菌 细胞定位 Triton Western 度条件 分析表 黄递酶 FNR Fd 实验 |
| 修稿时间： | 2005-01-21 |
Preliminary Studies on the 43-kD Protein in Phycobilisomes of Cyanobacterium Anacystis nidulans R-2 |
| |
| Abstract: | Phycobilisomes, containing a 43-kD protein, were isolated from Anacystis nidulans R-2. Dissociated phycobilisomes exhibited FNR diaphorase activity. Western blotting showed that the 43-kD protein could react with antibodies raised against Fd:NADP+ oxidoreductase. Analysis by Triton X-114 phase-partitioning indicated that the 43-kD protein could not enter into the detergent phase. Partial dissociation of phycobilisomes revealed no 43-kD protein in the fractions mainly containing rod components. |
| |
| Keywords: | Anacystis nidulans R-2 Phycobilisome Fd:NADP+ oxidoreductase |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
