Comparative Pathogenicity of <Emphasis Type="Italic">Lomentospora prolificans</Emphasis> (<Emphasis Type="Italic">Scedosporium prolificans</Emphasis>) Isolates from Mexican Patients |
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Authors: | Mariana Elizondo-Zertuche Alexandra M Montoya Efrén Robledo-Leal Idalia Garza-Veloz Ana L Sánchez-Núñez Raquel Ballesteros-Elizondo Gloria M González |
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Institution: | 1.Departamento de Microbiología, Facultad de Medicina,Universidad Autónoma de Nuevo León,Monterrey,Mexico;2.Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas,Universidad Autónoma de Nuevo León,San Nicolás de los Garza,Mexico;3.Laboratorio de Medicina Molecular, Unidad Académica de Medicina Humana y Ciencias de la Salud,Universidad Autónoma de Zacatecas,Zacatecas,Mexico;4.Departamento de Histología, Facultad de Medicina,Universidad Autónoma de Nuevo León,Monterrey,Mexico |
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Abstract: | We identified 11 Lomentospora prolificans isolates recovered from Mexican patients using phenotypic and molecular characteristics. The identification of isolates was assessed by internal transcribed spacer (ITS rDNA) sequencing. In vitro susceptibility to amphotericin B, fluconazole, voriconazole, posaconazole, caspofungin, anidulafungin and micafungin was determined according to Clinical and Laboratory Standards Institute (CLSI) procedures. Three isolates (07-2239, 11-2242 and 04-2673) were used to induce systemic infection in immunocompetent ICR mice. Survival and tissue burden studies were used as markers of pathogenicity. All of the strains were resistant to every antifungal tested with MIC’s for AmB (8–>8 µg/ml), VRC (16–>16 µg/ml), PSC (16–>16 µg/ml), FLC (64–>64 µg/ml) and echinocandins with MICs ≥8 µg/ml. One hundred, ninety and sixty percent of the infected mice with the strains 07-2239, 11-2242 and 04-2673 died during the study, respectively. Regarding tissue burden, the highest fungal load of the infected mice was detected in brain followed by spleen and kidney, regardless of the strain. |
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