Genotyping and Persistence of <Emphasis Type="Italic">Candida albicans</Emphasis> from Pregnant Women with Vulvovaginal Candidiasis |
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Authors: | Cecilia V Tapia Germán Hermosilla Paula Fortes Claudio Alburquenque Sergio Bucarey Hugo Salinas Paula I Rodas María Cristina Díaz Fabien Magne |
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Institution: | 1.Programa de Microbiología y Micología, ICBM, Facultad de Medicina,Universidad de Chile,Santiago,Chile;2.Laboratorio Clínica Dávila,Santiago,Chile;3.Escuela de Tecnología Médica,Universidad Mayor,Santiago,Chile;4.Departamento de Ciencias Biológicas Animales, Facultad de Ciencias Veterinarias y Pecuarias, Centro Biotecnológico Biovetec,Universidad de Chile,Santiago,Chile;5.Departamento de Obstetricia y Ginecología, Hospital Clínico,Universidad de Chile,Santiago,Chile;6.Center for Integrative Medicine and Innovative Science, Facultad de Medicina,Universidad Andrés Bello,Santiago,Chile;7.Laboratoire de Biologie,Conservatoire National des Arts et Métiers,Paris,France |
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Abstract: | Objective To study Candida albicans genotypes using RAPD and their susceptibility to fluconazole in healthy pregnant women and in vulvovaginal candidiasis (VVC) patients after topical treatment with clotrimazole.Methods Vaginal swabs were collected at t = 0 and t = 1 (1 month later) in pregnant women (control group, n = 33), and before (t = 0), at 1 month (t = 1) and at 2 months (t = 2) after clotrimazole treatment in pregnant women with VVC.Results Candida albicans was isolated in 30% of healthy pregnant women and 80% of patients with VVC. A high genetic heterogeneity was observed in C. albicans genotypes between individuals. In patients with VVC, topical antifungal treatment with clotrimazole was clinically effective, but only in a 62% C. albicans was eradicated. In patients in which C. albicans was not eradicated, this microorganism persisted for 1 or 2 months after the antifungal treatment. The persistent colonies were not associated with a specific genotype, but they were associated with higher MICs in comparison with colonies isolated from the control group.ConclusionsTherapy with topical clotrimazole, despite a good clinical outcome, could not eradicate completely C. albicans allowing the persistence of genotypes, with higher MICs to fluconazole. More studies with higher number of patients are needed to validate this preliminary finding. |
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