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The Mitotic Apparatus with Unusually Many Microtubules from Sea Urchin Eggs Treated by Hexyleneglycol
Authors:SACHIKO ENDO  MASARU TORIYAMA  HIKOICHI SAKAI
Affiliation:Department of Biophysics and Biochemistry, Faculty of Science, The University of Tokyo, Hongo, Tokyo 113, Japan
Abstract:The effect of hexyleneglycol on the structure of the mitotic apparatus was studied by light and electron microscopies. By treating sea urchin eggs at prometaphase and metaphase with hexyleneglycol, the mitotic apparatus was found to become remarkably decorated with unusually many astral microtubules which were conspicuously uniform in length. These microtubules appeared to be associated with the granular materials which are most likely microtubule initiating sites or microtubule-organizing centers.
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