Sensitivity enhancement of surface plasmon resonance biosensor with colloidal gold |
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Authors: | Kibong Choi Heeju Youn Kwangjoong Kim Jungdo Choi |
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Affiliation: | (1) GWSD-4-3, Agency for Defense Development, Yusung, P.O. Box 35, 305-600 Taejon, Korea;(2) Departmen of Biochemistry, Chungbuk National University, 361-763 Cheungju, Korea |
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Abstract: | We enhanced the sensitivity of surface plasmon resonance biosensor by the conversion of the real-time direct binding immunoassay into the sandwich immunoassay, in which colloidal gold particles coated with anti-mouse IgG was used. By the immobilization of anti-mouse IgG onto the carboxymethyl dextran surface of thin gold film, the direct binding of analyte (mouse IgG) onto the sensor chip, and the injection of colloidal gold particles coated with antimouse IgG, about 100 times of sensitivity enhancement was obtained. This result suggests that nanoparticles, which has a high refractive index, homogeneous ultrafine structure and capability of size control, would be applicable for the detection of very small quantity of biomaterial. |
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Keywords: | colloidal gold surface plasmon resonance sensitivity enhancement |
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