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Chemical and Olfactory Analysis of the Volatile Fraction of Ocimum gratissimum Concrete from Madagascar
Authors:Dr. Laure Gilles  Dr. Sylvain Antoniotti
Affiliation:1. Université Côte d'Azur, CNRS, Institut de Chimie de Nice, Parc Valrose, 06108 Nice cedex 2, France

Alysophil SAS Bio Parc, 850 bd Sébastien Brant BP 30170, 67405 Illkirch, France;2. Université Côte d'Azur, CNRS, Institut de Chimie de Nice, Parc Valrose, 06108 Nice cedex 2, France

Abstract:The chemical composition of the volatile fraction of Ocimum gratissimum concrete (romba) from Madagascar has been determined for the first time by GC/MS and GC-FID. A methyl cinnamate chemotype has been determined for this material, along with a set of compounds typical in essential oils and extracts from plants of the Ocimum genus. Variability was mostly observed on terpenes and terpenoids components. GC-O-MS was also used for a sensory evaluation of this material performed by a master perfumer. The chemical composition of this O. gratissimum extract was then compared with literature data to assess subtle differences between chemotypes of the same species and other species of the same genus within natural variability. A mapping illustrates the occurrence of the cinnamate chemotype in Eastern Africa, India and now Madagascar, while other origins generally present eugenol, thymol, camphor, or linalool chemotypes.
Keywords:complex natural substance  chemical analysis  natural variability  olfactometry  chemotypes
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