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New Meroterpenoids and Anti-Osteoclastogenic Polyketides from the Mangrove-Derived fungus Arthrinium sp. SCSIO 41306 |
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| Authors: | Yiwei Hu Xin Qi Weihao Chen Shengrong Liao Dehua Yang Mingwei Wang Yonghong Liu Yanhui Tan Junfeng Wang |
| Institution: | 1. CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology/Guangdong Key Laboratory of Marine Materia Medica/Innovation Academy of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301 P. R. China
University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049 P. R. China;2. CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology/Guangdong Key Laboratory of Marine Materia Medica/Innovation Academy of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301 P. R. China;3. The National Center for Drugs Screening, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203 P. R. China Research Center for Deepsea Bioresources, Sanya, 572025 P. R. China;4. CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology/Guangdong Key Laboratory of Marine Materia Medica/Innovation Academy of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301 P. R. China University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049 P. R. China Sanya Institute of Ocean Eco-Environmental Engineering, Sanya, 572000 P. R. China;5. State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin, 541001 P. R. China |
| Abstract: | Two new meroterpenoids, arthrinones A and B ( 1 and 2 ), along with six known compounds ( 3–8 ), were obtained from the fungus Arthrinium sp. SCSIO 41306. Comprehensive methods such as chiral-phase HPLC analysis and ECD calculations were applied to determine the absolute configurations. Griseofulvin ( 5 ), kojic acid ( 6 ), and 1H-indole-3-carboxaldehyde ( 8 ) showed inhibition of NF-κB in RAW 264.7 macrophages induced by lipopolysaccharide (LPS) with IC50 values of 22.21, 13.87 and 19.31 μM, respectively. In addition, griseofulvin ( 5 ) inhibited receptor activator of NF-κB ligand (RANKL)-induced osteoclast differentiation in a dose-dependent manner without visible evidence of cytotoxicity in bone marrow macrophages (BMMs). This is the first report on the activity of griseofulvin ( 5 ) to inhibit osteoclast formation (IC50 10.09±0.21 μM). |
| Keywords: | Arthrinium meroterpenoids NF-κB osteoclastogenesis PI3K |