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Lycopodium Alkaloids from Huperzia serrata and Their Anti-acetylcholinesterase Activities |
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| Authors: | Wen Li Hui-Hui Zhu Xi Shen Jin-Lin Tan Qing Tang Zhi-Peng Ling Hai-Yue Zhao Qiang Lin Hui Sun Hai-Peng Zhang Yao-Lan Li Guo-Cai Wang Yu-Bo Zhang |
| Institution: | 1. Institute of Traditional Chinese Medicine & Natural Products, Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou, 510632 P. R. China
These authors contributed equally to this work.;2. Guangdong Clinical Translational Center for Targeted Drug, Department of Pharmacology, School of Medicine, Jinan University, Guangzhou, 510632 P. R. China These authors contributed equally to this work.;3. Institute of Traditional Chinese Medicine & Natural Products, Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou, 510632 P. R. China;4. Hunan Institute for Drug Control, Changsha, Hunan, 410001 China;5. Guangdong Clinical Translational Center for Targeted Drug, Department of Pharmacology, School of Medicine, Jinan University, Guangzhou, 510632 P. R. China |
| Abstract: | One new fawcettimine-type alkaloid ( 1 ), one new miscellaneous-type alkaloid ( 2 ), four new lycodine-type alkaloids ( 3 – 6 ), and eight known ones ( 7 – 14 ) were isolated from the whole plants of Huperzia serrata. Their structures and absolute configurations were elucidated based on spectroscopic data, X-ray diffraction, ECD calculation and Mosher's method. Compound 1 was a rare C18N2-type Lycopodium alkaloid, possessing serratinine skeleton with an amide side chain in C-5. The absolute configuration of the 18-OH of compounds 4 – 6 were first determined by Mosher's method. Moreover, compounds 1 – 14 were assayed anti-acetylcholinesterase effect in vitro, and compound 7 showed significant anti-acetylcholinesterase activity with an IC50 value of 16.18±1.64 μM. |
| Keywords: | anti-acetylcholinesterase activity Huperzia serrata Lycopodium alkaloid |