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Quantification of Phenolic Component by LC-HESI-MS/MS and Evaluation of Antioxidant Activities of Crocus Ancyrensis (Ankara Çi?demi) Extracts Obtained with Different Solvents
Authors:Ömer Kayir  Hacer Do?an  Erol Alver  ?brahim Bilici
Institution:1. Scientific, Technical, Application and Research Center, Hitit University, 19030 Çorum, Turkey;2. Chemical Engineering, Faculty of Engineering, Hitit University, 19030 Çorum, Turkey
Abstract:In this study, Crocus ancyrensis was extracted from different parts of the plants with various solvents and their antioxidant activities and phenolic contents were investigated in detail for the first time. The highest amount of total phenolic substance in all parts of the plant was determined in the stigmaless flower. In the DPPH and FRAP methods, the highest antioxidant activity was obtained from the water extraction from the plant. Rutin is the highest detected by LC MS/MS. Stigmaless flower extract in all solvents is attributed to the component that contributes the most to antioxidant capacity. p-hydroxy benzoic acid was detected as the highest phenolic component after rutin. When the antioxidant activity results were examined, it was determined that the highest activity was in the water extract. As a result, it is evaluated that rutin and p-hydroxybenzoic acid in the plant contribute to the antioxidant capacity.
Keywords:antioxidant activity  Crocus ancyrensis  endemic  phenolic compounds  rutin
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