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Quantification of Phytochemicals and Metal Ions as well as the Determination of Volatile Compounds,Antioxidant, Antimicrobial and Antacid Activities of the Mimosa pudica L. Leaf: Exploration of Neglected and Under-Utilized Part |
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| Authors: | Ms Yashika Gandhi Dr Shyam Baboo Prasad Dr Vijay Kumar Dr Hemant Soni Hemant Rawat Dr Sujeet K Mishra Mrs Jyotika Grewal Dr Simranjeet Singh Dr Vaibhav Charde Mr Akhil Gupta Mr Sudhanshu Kumar Jha Mr Gagandeep Singh Dr Smriti Tandon Dr Akshada Mrkute Dr Praveen C Ramamurthy Dr Ch Venkata Narasimhaji Dr Arjun Singh Dr Ravindra Singh Narayan Srikanth Dr Rabinarayan Acharya Dr Thomas J Webster |
| Institution: | 1. Central Ayurveda Research Institute, Jhansi, Uttar Pradesh, 284003 India
Equal contribution Contribution: Conceptualization (equal), Data curation (equal), Formal analysis (equal), Validation (lead);2. Central Ayurveda Research Institute, Jhansi, Uttar Pradesh, 284003 India Equal contribution;3. Central Ayurveda Research Institute, Jhansi, Uttar Pradesh, 284003 India;4. Central Ayurveda Research Institute, Jhansi, Uttar Pradesh, 284003 India Equal contribution Contribution: Data curation (equal), ?Investigation (equal), Methodology (equal);5. Indian Institute of Sciences, Bangalore, 560012 India;6. Indira College Of Pharmacy Nanded, Maharashtra, 431606 India;7. Central Council for Research in Ayurvedic Sciences, New Delhi, 110058 India;8. School of Health Sciences and Biomedical Engineering, Hebei University of Technology, Tianjin, China School of Engineering, Saveetha University, Chennai, India Program in Materials Science, UFPI, Teresina, Brazil |
| Abstract: | Mimosa pudica L. (MP) is well-known plant in traditional medicinal system, especially in India. Unfortunately, leaves of MP are less explored. To determine the food and nutritional value of the neglected part of Mimosa pudica L. (MP), that is MP leaves, phytochemicals and metal ions of MP were quantified by newly developed HPLC and ICPOES-based methods. The content of phytochemicals observed using HPLC analysis for chlorogenic acid, catechin, and epicatechin was 141.823 (±8.171), 666.621 (±11.432), and 293.175 (±12.743) μg/g, respectively. Using GC/MS/MS analysis, fatty acid like oleic acid were identified. In ICP-OES analysis, a significant content of Na, K, Ca, Cu, Fe, Mg, Mn, and Zn was observed. The observed TPC and TFC for MP leaf extracts was 44.327 (±1.041) mg GAE/ g of wt. and 214.217 (±4.372) mg QCE/ g of wt., respectively. The DPPH assay depicted a strong antioxidant activity of MP leaf extracts with IC50 values of 0.796 (±0.081) mg/mL and a TEAC value of 0.0356 (±0.0003). A significant antacid activity (666 mg MP+400 mg CaCO3 >400 mg CaCO3 ?666 mg Gelusil) of MP leaves was noticed. The methanolic extract of MP leaves demonstrated anti-microbial activity against Staphylococcus aureus (15±2mm), Pseudomonas aeruginosa (12±2mm) and Escherichia coli (10±2mm). In silico studies confirmed the in vitro results obtained for antioxidant, antiacid, and anti-microbial activities. In addition, in silico studies revealed the anti-cancerous and anti-inflammatory potential of the MP leaves. In summary, this study demonstrated the medicinal significance of MP leaves and the conversion of agro-waste or the under-utilized part of MP into pharmaceutical potent materials. Consequently, the present study highlighted that MP leaves alone have medicinal importance with good nutritional utility and possess large promise in the pharma industry along with improving bio-valorization and the environment. |
| Keywords: | herbal Lajjalu Mimosa pudica phytochemicals polyphenols |