Evaluations of Anticancer Effects of Combinations of Cisplatin and Tirucallane-Type Triterpenes Isolated from Amphipterygium adstringens (Schltdl). |
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| Authors: | Lidia Díaz-Sánchez Alejandro Zentella-Dehesa Víctor Alberto Castro-Torres Noemi Silva-Jiménez Nadia Judith Jacobo-Herrera Mariano Martínez-Vázquez |
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| Institution: | 1. Instituto de Química, Universidad Nacional Autónoma de México, C. Exterior, C. Universitaria, Coyoacán, 04510, CDMX México (MMV);2. Departamento de Bioquímica, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán INCMNSZ., C. Vasco de Quiroga 15, Tlalpan, 14080 CDMX México (NJJH) |
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| Abstract: | The cytotoxic activity of combinations of masticadienonic (AMD) or 3αOH-hydroxy-masticadienonic (3αOH-AMD) acids with cisplatin (CDDP) was evaluated against PC3 prostate and HCT116 colon cancer cell lines. Combinations A (half the IC50 value), B (IC50 value), and C (twice the IC50 value) were tested at a 1 : 1 ratio. All AMD plus CDDP combinations demonstrated increased cytotoxic effect, as determined by the sulforhodamine B test, in both cell types. The best combination was B, which showed 93 % and 91 % inhibition of the proliferation of PC3 and HCT116 cells, respectively. It also increased apoptosis in the PC3 cell lines, as evaluated by flow cytometry. However, in vivo tests showed no additional activity from the AMD plus CDDP combinations. These results showed that the increased cytotoxic activity of the combinations in vitro did not reflect in vivo tests. All combinations of 3αOH-AMD plus CDDP exerted antagonistic effects in both cell types. |
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| Keywords: | antitumor cisplatin combinations cytotoxic antitumor cisplatin combinations cytotoxic triterpene triterpenes |
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