|
|||||
|
|
Propolis Enhances 5-Fluorouracil Mediated Antitumor Efficacy and Reduces Side Effects in Colorectal Cancer: An in Vitro and in Vivo Study |
|
| Authors: | Abdurrahim Kocyigit Eray Metin Guler Ezgi Durmus Vildan Betul Yenigun Ebru Kanimdan Zeynep Ozman Oznur Yasar Ahmet Ceyhan Goren Emine Rumeysa Hekimoglu Hasan Huseyin Oruc Gokhan Aydogdu |
| Affiliation: | 1. Bezmialem Vakif University Medical Faculty, Department of Medical Biochemistry, 34093 Fatih, Istanbul, Turkey;2. University of Health Sciences Turkey, Hamidiye School of Medicine, Department of Medical Biochemistry, Istanbul, Turkey;3. Bezmialem Vakif University Medical Faculty, Department of Medical Biochemistry, 34093 Fatih, Istanbul, Turkey Bezmialem Vakıf University Institute of Health Sciences, Medical Biochemistry, Turkey;4. Bezmialem Vakif University Medical Faculty, Department of Medical Biochemistry, 34093 Fatih, Istanbul, Turkey Istanbul University Institute of Science, Molecular Biology and Genetics, Turkey;5. Faculty of Pharmacy, Department of Basic Pharmaceutical Sciences, Bezmialem Vakif University, Istanbul, Turkey;6. Bezmialem Vakif University School of Medicine Department of Histology and Embryology, Istanbul, Turkey;7. Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Uludag University, Bursa, Turkey;8. Faculty of Veterinary Medicine Uludag University, Bursa, Turkey |
| Abstract: | In this study, we investigated the combined treatment of 5-fluorouracil (5-FU) and Anatolian propolis extract (PE) on colorectal cancer (CRC)using in vitro and in vivo studies. We exposed luciferase-transfected (Lovo-Luc CRC) cells and healthy colon cells (CCD-18Co) to varying concentrations of 5-FU and PE to assess their genotoxic, apoptotic, and cytotoxic effects, as well as their intracellular reactive oxygen species (iROS) levels. We also developed a xenograft model in nude mice and evaluated the anti-tumor effects of PE and 5-FU using various methods. Our findings showed that the combination of PE and 5-FU had selectivity against cancer cells, particularly at higher doses, and enhanced the anti-tumor effectiveness of 5-FU against colon CRC. The results suggest that PE can reduce side effects and increase the effectiveness of 5-FU through iROS generation in a dose-dependent manner. |
| Keywords: | propolis colorectal cancer 5-fluorouracil in vivo animal imaging system xenograft models |